Goat vitamin B12 (VB12) ELISA kit

产品详情

产品描述：

CUSABIO山羊维生素B12（VB12）酶联免疫检测试剂盒（货号：CSB-EQ028114GO），基于竞争法原理实现样本中VB12的定量分析，适用于检测血清、血浆及组织匀浆样本，检测灵敏度达1.56 pg/mL，线性范围为1.56-100 pg/mL，可精准测定微量VB12水平。维生素B12作为参与红细胞生成、DNA合成及神经系统功能的关键辅酶，其浓度监测对动物营养代谢研究具有重要意义。CUSABIO试剂盒采用高特异性抗体，在标准实验条件下可快速完成检测流程，适用于科研领域中对山羊VB12代谢机制的探索，如饲料营养评估、动物生理机能研究或疾病模型中VB12动态变化的分析。实验兼容多种生物样本，组织匀浆检测功能为动物组织水平研究提供便利。试剂盒严格进行批次质控，确保数据稳定性和重复性，为畜牧业、兽医学及基础科研领域的VB12相关研究提供可靠工具。
缩写：

VB12
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	85
	Range %	80-92
1:20	Average %	97
	Range %	90-105
1:40	Average %	97
	Range %	92-104
1:80	Average %	92
	Range %	86-98

回收率：

The recovery of goat VB12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.