Goat interleukin 12,IL-12/P40 ELISA KIT

Goat interleukin 12,IL-12/P40 ELISA KIT

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中文名称：

山羊白介素12(IL-12/P40)酶联免疫试剂盒
货号：

CSB-E12103G
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法，专为定量检测山羊血清、血浆、组织匀浆及细胞裂解液中的白介素12（IL-12/P40）而设计，其检测灵敏度达到9.38 pg/mL，线性范围覆盖9.38-600 pg/mL，可精准测定微量样本中该细胞因子的表达水平。IL-12作为由抗原呈递细胞分泌的关键免疫调节因子，其P40亚基在介导Th1细胞分化、增强自然杀伤细胞活性及调控细胞免疫应答中发挥重要作用。该试剂盒通过高度特异性的捕获抗体和检测抗体组合，结合显色系统实现目标蛋白的定量分析，适用于科研领域探究宿主抗感染免疫机制、疫苗效力评估或炎症性疾病模型中细胞因子网络的动态变化，例如可用于研究山羊布鲁氏菌病、口蹄疫等传染性疾病过程中的免疫激活状态，或在体外实验中评估免疫刺激剂对IL-12/P40分泌水平的影响。试剂盒包含预包被板条、标准品及全套实验组分，支持96孔板规格的灵活实验设计。
别名：

IL12B ELISA Kit; Interleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
缩写：

IL12B
Uniprot No.：

P68221
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

9.37 pg/mL-600 pg/mL
灵敏度：

2.34 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	86
	Range %	82-97
1:4	Average %	93
	Range %	85-102
1:8	Average %	95
	Range %	89-105

回收率：

The recovery of goat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	92	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
600	1.974	1.942	1.958	1.857
300	1.331	1.362	1.347	1.246
150	0.912	0.924	0.918	0.817
75	0.587	0.612	0.600	0.499
37.5	0.401	0.388	0.395	0.294
18.75	0.295	0.309	0.302	0.201
9.37	0.214	0.225	0.220	0.119
0	0.102	0.100	0.101

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
亚细胞定位：

Secreted.
蛋白家族：

Type I cytokine receptor family, Type 3 subfamily
数据库链接：

KEGG: chx:100860845

UniGene: Chi.38710

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.