Goat gonadotropin-releasing hormone,GnRH ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO山羊促性腺激素释放激素（GnRH）定量检测试剂盒，采用竞争性酶联免疫吸附法（ELISA）原理，专为科研实验设计。GnRH是下丘脑分泌的关键神经肽激素，通过调控垂体促性腺激素释放参与生殖系统功能调节，在动物生殖生理学及内分泌研究中具有重要价值。试剂盒检测灵敏度可达2.5 pg/mL，线性范围覆盖2.5-500 pg/mL，适用于血清、血浆及组织匀浆样本的检测。实验采用预包被特异性抗体的96孔板，通过生物素标记检测系统实现目标物定量分析，全程反应时间约3小时，操作便捷。该产品可广泛应用于山羊等反刍动物的生殖周期研究、繁殖调控机制探索、激素水平动态监测以及环境内分泌干扰物评估等科研场景。试剂盒组分包含标准品、检测抗体、显色底物等必要试剂，储存条件为2-8℃，有效期12个月，建议实验前通过预实验优化样本稀释比例以确保检测准确性。
缩写：

GnRH
Uniprot No.：

D3YJ02
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.5 pg/mL-500 pg/mL
灵敏度：

1.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	95
	Range %	88-102
1:4	Average %	94
	Range %	89-99
1:8	Average %	95
	Range %	89-103

回收率：

The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-102
EDTA plasma (n=4)	97	91-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.