Goat follicle-stimulating hormone,FSH ELISA Kit

Goat follicle-stimulating hormone,FSH ELISA Kit

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中文名称：

山羊促卵泡素(FSH)酶联免疫试剂盒
货号：

CSB-E13277G
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO山羊促卵泡素（FSH）酶联免疫检测试剂盒（货号：CSB-E13277G），采用竞争法原理实现定量分析，专为检测山羊血清、血浆样本中FSH水平而设计。FSH作为垂体前叶分泌的关键糖蛋白激素，在调控性腺发育、卵泡生长及生殖周期中发挥核心作用，其水平变化可反映动物生殖内分泌状态。本试剂盒检测范围为20-800 mIU/mL，通过预包被特异性抗体与样本中FSH及标记物的竞争结合反应，配合显色系统实现精准定量，操作流程标准化且重复性良好。适用于科研领域中对山羊繁殖生理机制、生殖疾病模型构建、畜牧业繁殖力优化等研究，例如发情周期动态监测、卵巢功能评估、激素干预效果分析等场景。试剂盒严格适配血清、血浆样本类型，可为生殖生物学、动物科学及兽医学研究提供可靠数据支持，满足实验室对山羊生殖健康相关激素检测的科研需求。
别名：

FSHBFollitropin subunit beta ELISA kit; Follicle-stimulating hormone beta subunit ELISA kit; FSH-B ELISA kit; FSH-beta ELISA kit; Follitropin beta chain ELISA kit
缩写：

FSHB
Uniprot No.：

Q8HY83
种属：

Capra hircus (Goat)
样本类型：

serum, plasma
检测范围：

20 mIU/mL-800 mIU/mL
灵敏度：

20 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	87-108
1:2	Average %	88
	Range %	82-98
1:4	Average %	95
	Range %	85-105
1:8	Average %	93
	Range %	84-100

回收率：

The recovery of goat FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	92	87-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
800	0.138	0.132	0.135
400	0.226	0.241	0.234
184	0.343	0.320	0.332
60	0.610	0.588	0.599
20	1.241	1.215	1.228
0	2.638	2.607	2.623

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Multiple-Pathway Synergy Alters Steroidogenesis and Spermatogenesis in Response to an Immunocastration Vaccine in Goat Y Ding,/,2023

功能：

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

UniGene: Chi.38676

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.