Goat Tumor necrosis factor α,TNF-α ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO山羊肿瘤坏死因子α（TNF-α）酶联免疫检测试剂盒（货号：CSB-E09811g），采用夹心法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中TNF-α的浓度。TNF-α作为重要的促炎细胞因子，参与调节免疫应答、炎症反应及细胞凋亡等生理病理过程，在感染性疾病、自身免疫病等研究中具有重要价值。试剂盒检测范围为15.6 pg/mL至1000 pg/mL，覆盖山羊体内TNF-α的常见表达水平，可精准检测微量样本中的靶蛋白含量。该试剂盒操作便捷，配套标准品和预包被抗体，支持科研人员开展山羊炎症模型建立、免疫机制研究、疫苗效力评估等实验，为畜牧兽医研究、动物疾病模型开发及生物制品质量控制提供可靠工具。需注意本产品仅限科学研究使用，实验需按说明书规范操作以确保结果准确性。
别名：

TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
缩写：

TNF
Uniprot No.：

P13296
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-91
1:2	Average %	98
	Range %	91-103
1:4	Average %	99
	Range %	95-104
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of goat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-97
EDTA plasma (n=4)	97	92-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
基因功能参考文献：
1. Data indicate that three single-nucleotide polymorphisms were identified in tumor necrosis factor-alpha (TNF-alpha) gene. PMID: 25966128
亚细胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
数据库链接：

KEGG: chx:100861232

UniGene: Chi.11711