Goat Interleukin 2(IL-2) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CSB-E12965G山羊白介素2（IL-2）酶联免疫试剂盒采用双抗体夹心法原理，专为定量检测山羊血清、血浆及组织匀浆样本中的IL-2含量而设计。IL-2是由活化T淋巴细胞分泌的关键细胞因子，在调节免疫细胞增殖、分化及维持免疫稳态中发挥重要作用，是研究感染性疾病、自身免疫机制及疫苗效力评估的重要靶标。本试剂盒检测灵敏度为15.6 pg/mL，线性检测范围为15.6-1000 pg/mL，可精准检测低浓度样本，适用于科研领域中对山羊免疫相关疾病的机制探索、炎症模型分析或疫苗开发中细胞免疫应答的定量评估。实验操作需使用50-100 μL样本，通过预包被捕获抗体与样本中IL-2结合后，再经酶标检测抗体形成复合物，最终通过显色反应实现目标蛋白的定量分析。该产品严格验证了抗体特异性与批次稳定性，为兽医研究、比较免疫学及畜牧领域中的免疫调控研究提供可靠工具。
别名：

IL2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写：

IL2
Uniprot No.：

P36835
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	98
	Range %	94-102
1:4	Average %	102
	Range %	94-107
1:8	Average %	94
	Range %	87-98

回收率：

The recovery of goat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-102
EDTA plasma (n=4)	93	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
亚细胞定位：

Secreted.
蛋白家族：

IL-2 family
数据库链接：

UniGene: Chi.38711