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  4. Goat Interleukin 1β(IL-1β) ELISA Kit

Goat Interleukin 1β(IL-1β) ELISA Kit

文献引用 (4) 说明书下载
  • 中文名称：
    山羊白介素1β(IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E16568G
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO山羊白介素1β（IL-1β）酶联免疫检测试剂盒（货号：CSB-E16568G），采用双抗体夹心法定量检测血清、血浆及组织匀浆液样本中IL-1β的浓度。IL-1β是一种重要的促炎性细胞因子，广泛参与炎症反应、免疫调节及细胞信号传导过程，在感染性疾病、自身免疫疾病及应激反应研究中具有重要科研价值。试剂盒检测灵敏度高，线性范围为3.12-200 pg/mL，可精准检测微量样本中IL-1β的表达水平。实验采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配合标准品建立标准曲线完成定量分析。该试剂盒适用于动物模型研究、炎症机制探索、免疫调控实验等科研领域，能够为病原体感染评估、炎症因子网络分析、免疫干预效果验证等基础研究提供可靠数据支持，操作流程规范且重复性良好，需自备酶标仪等基础实验设备。
  • 别名：
    IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
  • 缩写：
    IL-1β
  • Uniprot No.：
  • 种属：
    Capra hircus (Goat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-94
    1:2Average %90
    Range %82-97
    1:4Average %92
    Range %85-100
    1:8Average %95
    Range %90-103
  • 回收率：
    The recovery of goat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-98
    EDTA plasma (n=4)9993-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.112 2.052 2.082 1.951
    1001.576 1.654 1.615 1.484
    501.039 0.984 1.012 0.881
    250.587 0.549 0.568 0.437
    12.50.384 0.397 0.391 0.260
    6.250.292 0.269 0.281 0.150
    3.120.208 0.199 0.204 0.073
    00.127 0.134 0.131 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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IL1B Proteins

靶点详情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    UniGene: Chi.15482

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |