Goat Interleukin 10,IL-10 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO山羊白介素10（IL-10）酶联免疫检测试剂盒（货号：CSB-E09809g），采用双抗体夹心法原理实现定量检测，适用于分析血清、血浆及组织匀浆样本中的IL-10含量。IL-10是一种关键的抗炎细胞因子，在免疫调节中通过抑制促炎因子释放和调控免疫细胞功能维持机体稳态，其表达水平与炎症反应、感染性疾病及免疫相关研究密切相关。试剂盒检测范围覆盖31.25 pg/mL至2000 pg/mL，最低检测灵敏度为31.25 pg/mL，标准曲线呈现良好的线性关系，可精准测定样本中IL-10的浓度变化。该试剂盒提供预包被96孔板及全套检测组分，操作流程包括样本孵育、洗涤、显色及终止反应步骤，适用于科研领域研究山羊模型中的免疫应答机制、炎症性疾病进展评估或疫苗开发中的免疫调节分析。实验全程需使用酶标仪在450 nm波长下读取吸光度值，建议配套设置标准品复孔及空白对照以保障检测准确性。本产品仅供科学研究使用，为动物免疫学研究提供可靠的数据支持。
缩写：

IL-10
Uniprot No.：

A7Y3X5
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	82
	Range %	80-85
1:2	Average %	102
	Range %	97-107
1:4	Average %	89
	Range %	84-93
1:8	Average %	107
	Range %	103-111

回收率：

The recovery of goat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	85	80-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.