Goat Albumin (Alb)ELISA Kit

产品详情

产品描述：

CUSABIO山羊白蛋白（Alb）酶联免疫检测试剂盒（货号：CSB-E15758G），采用竞争法定量分析血清、血浆及组织匀浆中的Alb浓度。白蛋白是血浆中含量最丰富的蛋白质，由肝脏合成，在维持胶体渗透压、物质运输及代谢调节中发挥重要作用。该试剂盒检测范围覆盖0.17 μg/mL至350 μg/mL，适用于低浓度样本检测及高浓度样本梯度稀释分析，实验操作中需自备酶标仪及移液设备。基于竞争法原理，试剂盒通过样本中的Alb与标记抗原竞争结合包被抗体，经显色反应后测定吸光度值，实现目标蛋白的精准定量。本产品可应用于动物营养代谢研究、肝功能评估实验、疾病模型构建中的生物标志物分析等科研领域，为山羊生理病理机制探索及畜牧业相关研究提供可靠工具，实验结果需结合标准曲线进行数据计算，实验全程需严格遵循说明书操作规范。
别名：

ALB ELISA Kit; Serum albumin ELISA Kit; Fragments ELISA Kit
缩写：

ALB
Uniprot No.：

P85295
种属：

Capra hircus (Goat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.17 μg/mL-350 μg/mL
灵敏度：

0.17 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat Alb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	91-105
1:2000	Average %	90
	Range %	88-97
1:4000	Average %	90
	Range %	83-100
1:8000	Average %	95
	Range %	82-99

回收率：

The recovery of goat Alb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-105
EDTA plasma (n=4)	96	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
亚细胞定位：

Secreted.
蛋白家族：

ALB/AFP/VDB family
组织特异性：

Plasma.