Fish trypsin ELISA Kit

产品详情

产品描述：

CUSABIO鱼胰蛋白酶（trypsin）酶联免疫检测试剂盒（货号：CSB-EL018811FI），采用竞争法原理实现定量分析，适用于鱼类血清、血浆及组织匀浆样本中胰蛋白酶含量的精准检测。胰蛋白酶作为鱼类消化系统中的关键蛋白水解酶，参与蛋白质分解及免疫调节过程，其水平变化可为水产动物消化生理研究、疾病模型构建及营养代谢评估提供重要指标。试剂盒检测灵敏度为0.312 ng/mL，线性范围覆盖0.312-20 ng/mL，预包被的微孔板通过竞争结合样本中的胰蛋白酶与标记物，结合HRP酶联反应显色系统，实现目标物的稳定定量。包含标准品、检测抗体、酶结合物、终止液等完整组分，支持实验室开展鱼类消化系统功能研究、水产养殖基础科研以及环境因子对鱼类生理影响的毒理学评估，为科研人员提供标准化、高特异性的检测工具。
别名：

Trypsin-1 ELISA kit; EC 3.4.21.4 ELISA kit; Trypsin I ELISA kit
缩写：

PRSS1
Uniprot No.：

P35031
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	101
	Range %	95-105
1:40	Average %	91
	Range %	84-95
1:80	Average %	106
	Range %	100-111
1:160	Average %	104
	Range %	97-108

回收率：

The recovery of fish trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-100
EDTA plasma (n=4)	102	96-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.