Fish gonadotropin-releasing hormone,GnRH ELISA Kit

Fish gonadotropin-releasing hormone,GnRH ELISA Kit

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中文名称：

鱼类促性腺激素释放激素(GnRH)酶联免疫试剂盒
货号：

CSB-E08810f
规格：

96T/48T
价格：

￥4800/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO鱼类促性腺激素释放激素（GnRH）酶联免疫检测试剂盒（货号：CSB-E08810f），采用竞争法原理实现定量分析，适用于科研领域中对鱼类血清、血浆、细胞培养上清液、组织匀浆及细胞裂解液等样本中GnRH含量的精准检测。GnRH作为下丘脑分泌的关键神经肽，通过调控垂体促性腺激素的释放，在鱼类生殖调控、性腺成熟及繁殖周期中发挥核心作用。该试剂盒检测灵敏度高，线性范围覆盖0.5 ng/mL至20 ng/mL，可稳定识别天然与重组GnRH分子，操作流程包含预包被板、生物素标记抗体及标准品等核心组分，实验全程约需3-4小时完成。科研人员可借助本试剂盒开展鱼类生殖生理机制研究、水产养殖中人工繁殖技术优化、环境激素干扰评估等实验，例如监测不同发育阶段或环境胁迫下鱼类GnRH的动态变化，分析内分泌调控网络对繁殖行为的影响，为水生生物资源保护及可持续养殖提供数据支持。
缩写：

GnRH
Uniprot No.：

Q5Y835
种属：

Fish
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：

0.5 ng/mL-20 ng/mL
灵敏度：

0.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-104
1:2	Average %	94
	Range %	85-103
1:4	Average %	95
	Range %	83-102
1:8	Average %	92
	Range %	87-101

回收率：

The recovery of fish GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	94	89-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.134	0.140	0.137
10	0.232	0.242	0.237
4.6	0.398	0.409	0.404
1.5	0.714	0.687	0.701
0.5	1.293	1.266	1.280
0	2.711	2.740	2.726

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Reversible Sterilization of Channel Catfish via Overexpression of Glutamic Acid Decarboxylase Gene Z Ye,Animals,2024
Effects of gonadotropin inhibitory hormone or gonadotropin-releasing hormone on reproduction-related genes in the protandrous cinnamon clownfish, Amphiprion melanopus Choi Y J.et al,General and Comparative Endocrinology,2016

■ 常见问题解答

Fish have over a dozen different forms of GnRHs. I would llike to know for which fish GnRH form is this assay designed before placing an order.

Thanks for your inquiry.
This kit is designed against Zebrafish. Pls refer to: http://www.uniprot.org/uniprot/Q5Y835

If the gene mentioned in the data is indeed gnrh2, the assay cannot be specific for zebrafish or any other fish species. All vertebrate animals, including humans, have gnrh2 gene with mostly unknown function. The GnRH of interest in zebrafish is salmon GnRH or gnrh3 gene. I have to think about it. This kit may not be any better for zebrafish than those for mammalian GnRH because gnrh2 gene encodes for exactly the same functional peptide in all vertebrates.

Do you have data which shows this kit is specific to zebrafish? Could you perhaps send the COA?

Thanks for your inquiry.
GnRH has different isoforms：GnRH，GnRH2 and GnRH3. GnRH-2 is very conservative in different species of fish.
Pls kindly refer to: https://www.nature.com/articles/srep03676.
CSB-E08810f is designed against zebrafish. We tested undiluted serum and plasma samples before and the test value is 0.5--1.5ng/ml.
I will send you COA by email.
Pls let me know if you have any further questions. Thank you.

Thanks for your response. Based on the literature you provided it seems GnRH2 is not only conserved among fish species but among all vertebrates.
Since I am curious what the difference is between this kit and your kits from other species. Since the antigen is so highly conserved couldn't I use any of your other GnRH kits instead?

Although GnRH2 is highly conserved, different types of animals have different matrix.
Our kit for fish is designed against fish. For different animals, the raw materials used in the kits are different.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.