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  4. Fish Superoxide Dismutase(SOD)ELISA kit

Fish Superoxide Dismutase(SOD)ELISA kit

文献引用 (69) 说明书下载
  • 中文名称：
    鱼超氧化物歧化酶(SOD)酶联免疫试剂盒
  • 货号：
    CSB-E15929Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO鱼类超氧化物歧化酶（SOD）定量检测试剂盒（货号：CSB-E15929Fh），适用于血清、血浆及组织匀浆样本分析，检测灵敏度达1.25 ng/mL，线性范围为1.25-20 ng/mL。SOD作为生物体内关键的抗氧化酶，通过催化超氧阴离子自由基的歧化反应维持氧化还原平衡，其活性水平可反映鱼类在环境胁迫、病理状态或营养代谢中的抗氧化能力。CUSABIO试剂盒采用竞争性酶联免疫吸附法（ELISA）原理，通过样本中的SOD与标记物竞争结合特异性抗体，结合显色反应强度实现目标物定量分析。该产品可为鱼类生理学、环境毒理学及水产养殖研究提供可靠工具，例如评估污染物暴露对鱼类氧化应激的影响、监测养殖水体环境压力下的生物标志物变化，或探究营养干预对鱼类抗氧化系统的调控机制。试剂盒包含预包被板、标准品、检测抗体及显色底物等完整组分，操作流程标准化，适用于实验室批量样本的高通量检测需求。
  • 别名：
    sod1 ELISA Kit; cuzn ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
  • 缩写：
    SOD
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.25 ng/mL-20 ng/mL
  • 灵敏度：
    0.625 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 92-98  
    1:200 Average % 103  
    Range % 100-106  
    1:400 Average % 98  
    Range % 94-102  
    1:800 Average % 92  
    Range % 88-96  
  • 回收率：
    The recovery of fish SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 96-103  
    EDTA plasma (n=4) 89 85-93  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.092 0.099 0.096    
    10 0.143 0.152 0.148    
    5 0.245 0.223 0.234    
    2.5 0.588 0.575 0.582    
    1.25 1.397 1.402 1.400    
    0 2.214 2.352 2.283    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

I use the SOD kit(fish) after centrifuge the standard vial at 6000~10000rpm for 30s before opening 2:56 pm
i must pipetting standard vial or not?

A:
Thanks for your inquiry. The standard in this kit is 10 times concentrated and it requires dilutions.For the specific cooperations,pls refer to the "Standard" part stated on page 9 of the manual.I send the manual to you by e-mail. Pls let me know if you have any further questions. Thank you.
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靶点详情

  • 最新研究进展：
    SOD是一种抗氧化酶，它能够清除自由基，防止氧化损伤。最新的研究表明，SOD不仅在维持细胞内氧化还原平衡中发挥重要作用，还可能在其他生理和病理过程中发挥作用。例如，SOD的缺失与许多疾病有关，包括癌症、糖尿病和神经系统疾病。此外，SOD也被认为可能与衰老和长寿有关，一些研究表明SOD的增加可能有助于延缓衰老进程。
  • 功能：
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • 基因功能参考文献：
    1. fenofibrate almost completely abolished GM-induced reactive oxygen species generation, which seemed to be mediated at least in part by the restoration of the expression of PPARalphadependent antioxidant enzymes, including catalase and superoxide dismutase (SOD)-1. PMID: 28290603
    2. The earliest event in the pathophysiology of amyotrohic lateral sclerosis in the mutant sod1 zebrafish model involves neuronal stress in inhibitory interneurons, resulting from mutant Sod1 expression. PMID: 23281025
    3. A hierarchic gene expression of copper homeostatic genes was demonstrated between atp7a, sp1 and sod1 in zebrafish. PMID: 21167140
    4. depresses cathepsin L activity stimulated by free radicals and prevents otic complications associated with bone erosion PMID: 11771045
    5. This isoform, when expressed in PC-12 cells affects t-butylhydroperoxide-induced apoptosis differentially from its isoenzyme. PMID: 12551919
    6. Copper/zinc superoxide dismutase was cloned from the zebrafish ( Danio rerio). Evidence is presented that SOD protects against paraquat toxicity in fish. PMID: 12876653
    7. Glia maturation factor-null cells ahow a concurrent decrease in CuZnSOD astrocytes. PMID: 15260123

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Cu-Zn superoxide dismutase family
  • 数据库链接：

    KEGG: dre:30553

    STRING: 7955.ENSDARP00000064375

    UniGene: Dr.75822

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |