Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO鱼类催乳素（PRL/LTH）定量检测专用ELISA试剂盒，采用竞争法检测原理，适用于血清、血浆及组织匀浆样本的科研分析。催乳素在鱼类生理活动中参与调控生殖周期、渗透压平衡及生长代谢等关键过程，对水产动物内分泌研究具有重要意义。试剂盒检测范围为10-4000 μIU/mL，最低检测限为10 μIU/mL，实验需提前对组织样本进行匀浆处理并离心取上清液。该试剂盒通过预包被特异性抗体，待测样本中的PRL/LTH与酶标记抗原竞争结合抗体位点，经显色反应后检测吸光度值，其信号强度与目标物浓度呈负相关，可实现定量分析。广泛应用于鱼类繁殖生理学研究、环境内分泌干扰物评估、水产养殖过程中激素水平监测等科研场景，为水生生物内分泌机制探索提供可靠工具。试剂盒包含预包被板、标准品、检测抗体及全套实验试剂，操作流程标准化，适用于实验室批量样本检测需求。
别名：

prl1 ELISA Kit; Prolactin-1 ELISA Kit; PRL-188 ELISA Kit; Prolactin I ELISA Kit
缩写：

PRL/LTH
Uniprot No.：

P09319
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 μIU/mL-4000 μIU/mL
灵敏度：

10 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-100
1:2	Average %	95
	Range %	86-104
1:4	Average %	90
	Range %	85-95
1:8	Average %	96
	Range %	92-108

回收率：

The recovery of fish PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-102
EDTA plasma (n=4)	95	88-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.