Fish Interleukin-8,IL-8 ELISA Kit

产品详情

产品描述：

CUSABIO鱼类白介素8（IL-8/CXCL8）酶联免疫检测试剂盒（货号：CSB-E12877Fh），采用竞争法定量分析原理，专为科研领域设计，适用于血清、血浆样本中IL-8/CXCL8蛋白的精准检测。IL-8作为趋化因子家族核心成员，在鱼类炎症反应、免疫调控及病原体防御中发挥重要作用，其表达水平可作为评估机体免疫状态及炎症进程的关键指标。试剂盒检测灵敏度高，线性范围为31.25-500 pg/mL，能够稳定识别样本中微量目标蛋白，满足实验室对鱼类疾病模型构建、免疫调节机制研究或环境胁迫响应分析的定量检测需求。实验操作采用标准ELISA流程，包含预包被抗体及优化反应体系，确保数据重复性与可靠性，配套试剂组分齐全，支持科研人员高效开展水产动物免疫学、病理学等基础研究，为揭示鱼类先天免疫机制提供可靠工具。
缩写：

IL8/CXCL8
Uniprot No.：

Q6F4N2
种属：

Fish
样本类型：

serum, plasma
检测范围：

31.25 pg/mL-500 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	92-97
1:2	Average %	95
	Range %	85-100
1:4	Average %	93
	Range %	89-101
1:8	Average %	95
	Range %	86-100

回收率：

The recovery of fish IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	85-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.