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  4. Fish Interleukin 1β (IL- 1β) ELISA Kit

Fish Interleukin 1β (IL- 1β) ELISA Kit

文献引用 (13) 说明书下载
  • 中文名称：
    鱼白介素1β(IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E13259Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO鱼类白介素1β（IL-1β）酶联免疫检测试剂盒（货号：CSB-E13259Fh），采用双抗体夹心法原理实现定量分析，适用于科研领域中对鱼类血清、血浆及组织匀浆样本中IL-1β蛋白含量的精准检测。IL-1β是鱼类免疫系统中重要的促炎细胞因子，参与调控炎症反应、免疫应答及组织修复等生理过程，其表达水平可作为评估鱼类感染性疾病、应激反应及免疫调控机制的重要指标。该试剂盒检测灵敏度为3.12 pg/mL，线性范围覆盖3.12-200 pg/mL，能够稳定检测低丰度目标蛋白，实验操作全程约需4.5小时，包含预包被捕获抗体、生物素标记检测抗体及标准品等核心组分。本产品广泛应用于水产动物免疫学研究、鱼类病原感染模型构建、疫苗效力评估以及水产养殖环境胁迫对机体免疫功能影响等科研场景，为深入解析鱼类先天免疫机制提供可靠工具。实验需自备酶标仪、移液器等基础设备，所有组分需按说明书要求保存于2-8℃或-20℃环境以确保活性稳定。
  • 别名：
    il1bInterleukin-1 beta ELISA Kit
  • 缩写：
    IL-1β
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IL- 1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 85
    Range % 82-89
    1:2 Average % 94
    Range % 90-99
    1:4 Average % 104
    Range % 97-108
    1:8 Average % 99
    Range % 94-106
  • 回收率：
    The recovery of fish IL- 1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-102
    EDTA plasma (n=4) 89 82-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    200 2.989 2.778 2.884 2.776
    100 2.467 2.432 2.450 2.342
    50 1.774 1.866 1.820 1.712
    25 0.956 0.932 0.944 0.836
    12.5 0.436 0.456 0.446 0.338
    6.25 0.258 0.270 0.264 0.156
    3.12 0.156 0.155 0.156 0.048
    0 0.105 0.111 0.108 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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靶点详情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
  • 基因功能参考文献：
    1. Major changes due to graded levels of olive leaf extract in the diets were observed in blood biochemical parameters. TNFalpha, IL1-beta and IL-8 gene expressions were significanlty up-regulated in OLE 0.1% group compared with others. PMID: 29733961
    2. The predicted 3D structures of IL-1beta, TNF-alpha, and IL-8 from Oncorhynchus mykiss, Dicentrarchus labrax, and Chionodraco hamatus are reported and the differences between them and their mammalian counterparts investigated. PMID: 21184658
  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Cytoplasmic vesicle, autophagosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 组织特异性：
    All three isoforms are expressed in gill, kidney, liver and spleen. Isoform 1 is also expressed in blood. None of the isoforms are expressed in brain.
  • 数据库链接：

    UniGene: Omy.11696

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |