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  4. Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

文献引用 (33) 说明书下载
  • 中文名称：
    鱼胰岛素样生长因子1(IGF-1)酶联免疫试剂盒
  • 货号：
    CSB-E12122Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO鱼胰岛素样生长因子1（IGF-1）酶联免疫检测试剂盒（货号：CSB-E12122Fh），采用竞争法原理实现定量分析，适用于检测鱼类血清、血浆及组织匀浆样本中IGF-1的浓度水平。IGF-1是调控鱼类生长、代谢和免疫功能的关键多肽因子，其表达水平与个体发育、营养状态及环境适应密切相关。试剂盒检测范围为25-2000 pg/mL，通过预包被特异性抗体实现目标物捕获，样本中的IGF-1与标记的竞争物共同结合抗体，经显色反应后通过标准曲线计算浓度值。该试剂盒可为水产动物生理研究、养殖管理优化以及环境胁迫响应机制探索提供可靠工具，特别适用于实验室条件下鱼类生长性能评估、饲料效应分析以及激素调控网络研究，实验全程无需复杂设备，配套试剂与耗材完备，3小时内即可完成检测流程。
  • 别名：
    igf1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
  • 缩写：
    IGF-1
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    25 pg/mL-2000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-98  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 102  
    Range % 98-106  
    1:8 Average % 87  
    Range % 82-93  
  • 回收率：
    The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 88 82-94  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2000 0.341 0.343 0.342    
    750 0.476 0.484 0.480    
    375 0.682 0.636 0.659    
    125 1.067 1.072 1.070    
    25 1.488 1.567 1.528    
    0 1.955 1.995 1.975    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

I want to use Fish Igf-1 ELISA kit for fish's liver homogenate.
In the protocol of the kit, for tissue homogenates, after homogenizing, stored overnight at -20oC. After 2 freeze-thaw cycles, centrifuge the homogenates.I do not understand too much about this. It means I have wait for 2 days to homogenate 2 sample (homogenate-> store -20oC-> thaw-> store 20oC -> thaw-> centrifuge)? Can you explain for me. Thanks!

A:
Thanks for your inquiry.Regarding tissue sample,pls store the sample which has been treated by PBS at -20 degrees overnight.After that,take the sample at room temperature and let it be thawed freely (the first freeze thaw cycle).Then freeze it at -80 degrees or store it at -20 degrees overnight.Then take it at temperature and let it be thawed freely.This is the 2nd freeze-thaw cycle.Regarding the later procedures,pls follow the manual.
If any further questions,pls let me know.
Q:

Can you let me know in which buffer the samples should be diluted?
This is not written in de manual!

A:
Thanks for your inquiry.
CSB-E12122Fh is no need to dilute generally.
So there is no sample diluent included in this kit.Pls let me know if you have any further questions. Thank you.
Q:

In the current protocol nothing is mentioned about an extraction of IGF-1 from binding proteins. This is generally a necessary procedure to properly measure IGF-1. Is an extraction, and if so which, recommended by Cusabio? Is this procedure in the most recent protocol?

A:
Thanks for your inquiry. There is no need to do extraction of IGF-1 from binding proteins for this kit.
Because this kit employs the competitive inhibition enzyme immunoassay technique and detects the total IGF1. Pls let me know if you have any further questions. Thank you.
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靶点详情

  • 功能：
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.
  • 基因功能参考文献：
    1. Nutrient availability, IGF-I, and genetic variation affect weight loss, in part through alterations of proteolytic pathways in rainbow trout. PMID: 19726716
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：

    UniGene: Omy.7104

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |