Fish Insulin-like growth factor II(IGF2) ELISA kit

中文名称：

鱼胰岛素样生长因子II(IGF2)酶联免疫试剂盒
货号：

CSB-EL011088FI
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
靶点详情

产品详情

产品描述：

CUSABIO鱼类胰岛素样生长因子II（IGF2）定量检测试剂盒（货号：CSB-EL011088FI），基于竞争性酶联免疫吸附法原理开发，适用于鱼类血清或血浆样本中IGF2蛋白的精确检测。IGF2作为鱼类重要的生长调节因子，在个体发育、组织修复及代谢调控中发挥关键作用，其水平变化与生长性能、营养状态及环境适应性密切相关。该试剂盒检测灵敏度高，线性范围为62.5-1000 pg/mL，可满足不同浓度样本的检测需求，标准品采用梯度稀释设计确保定量准确性。实验操作流程经过优化，96孔板预包被特异性抗体，通过生物素标记抗原与样本中IGF2竞争结合位点，配合显色反应实现靶标定量分析，全程约需3.5小时完成。本试剂盒适用于水产养殖研究、鱼类生理学实验、生长调控机制探究等科研领域，特别适用于评估饲料营养配比、环境胁迫对鱼类生长的影响或基因工程模型的生物标志物分析。产品配套提供完整标准品与缓冲液体系，具有批次稳定性强、交叉反应率低的特点，为实验室开展鱼类内分泌研究提供可靠工具。
别名：

igf2Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Erythrotropin ELISA Kit
缩写：

IGF2
Uniprot No.：

Q02816
种属：

Fish
样本类型：

serum, plasma
检测范围：

62.5 pg/mL-1000 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	87-102
1:200	Average %	95
	Range %	91-99
1:400	Average %	96
	Range %	88-106
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of fish IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	93	85-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.085	0.089	0.087
500	0.192	0.205	0.199
250	0.485	0.489	0.487
125	0.942	0.937	0.940
62.5	1.613	1.572	1.593
0	2.595	2.624	2.610

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

产品评价

靶点详情

功能：

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for integrin which is required for IGF2 signaling.
亚细胞定位：

Secreted.
蛋白家族：

Insulin family
数据库链接：

UniGene: Omy.11849

Fish Insulin-like growth factor II(IGF2) ELISA kit

产品详情

产品评价

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息