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中文名称:鱼胰岛素(INS)酶联免疫试剂盒
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货号:CSB-E12123Fh
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:鱼胰岛素(INS)是调控鱼类糖代谢的关键激素,其结构与哺乳动物胰岛素存在种属差异,因此在鱼类代谢研究中需采用特异性检测工具。本试剂盒(CSB-E12123Fh)基于竞争法ELISA原理开发,可定量分析血清、血浆及组织匀浆样本中的鱼胰岛素水平,检测灵敏度达100 pg/mL,线性范围为100-1600 pg/mL,满足科研场景中宽浓度范围的检测需求。试剂盒采用高特异性抗体,能有效区分鱼类INS与其他物种类似蛋白,确保检测结果准确性。该产品适用于鱼类生理学研究,例如评估不同生长阶段、营养状态或环境胁迫(如水温、盐度变化)对胰岛素分泌的影响,也可用于水产养殖中鱼类代谢疾病模型的机制探索。实验操作简便,仅需常规酶标仪即可完成检测,配套试剂包含标准品、预包被板及显色系统,支持批量样本的高效分析,为鱼类内分泌研究提供可靠工具。
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缩写:
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Uniprot No.:
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种属:Fish
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样本类型:serum, plasma, tissue homogenates
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检测范围:100 pg/mL-1600 pg/mL
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灵敏度:100 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Metabolism
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-100 1:2 Average % 98 Range % 92-103 1:4 Average % 85 Range % 80-89 1:8 Average % 91 Range % 83-99 -
回收率:
The recovery of fish INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 88-98 EDTA plasma (n=4) 92 90-95 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 1600 0.178 0.189 0.184 800 0.322 0.340 0.331 400 0.610 0.634 0.622 200 1.257 1.230 1.244 100 2.335 2.475 2.405 -
数据处理:
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货期:3-5 working days
引用文献
- Dietary functional palatability enhancer improved growth and appetite in largemouth bass (Micropterus salmoides) fed a reduced fish meal diet X Zhi, Y Gu, W Zhao, S Xie, Y Bao, S Morais, D Li,Aquaculture Reports,2025
- Vitamin D regulates glucose metabolism in zebrafish (Danio rerio) by maintaining intestinal homeostasis R Shao,The Journal of nutritional biochemistry,2024
- Vitamin D regulates insulin pathway and glucose metabolism in zebrafish (Danio rerio) R Shao,FASEB journal,2023
- Sequence identification and expression characterization of leptin in the spotted scat, Scatophagus argus WX Li,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
- Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance Z Zhao,bioRxiv,2023
- Synergetic effects of concurrent chronic exposure to a mixture of OCPs and high-fat diets on type 2 diabetes and beneficial effects of caloric restriction in female zebrafish HyojinLee,Journal of Hazardous Materials,2022
- The feedback regulation of carbohydrates intake on food intake and appetite in grass carp (Ctenopharyngodon idella) XC Yuan,Fish Physiol Biochem,2021
- Effects of acute hyperglycaemia stress on indices of glucose metabolism and glucose transporter genes expression in cobia (Rachycentron canadum) Ye G, et al,Aquaculture Research,2019
- Comparative analysis of glucose metabolism responses of large yellow croaker Larimichthys crocea fed diet with fish oil and palm oil Wang Q,Fish Physiology and Biochemistry,2019
- High level of dietary soybean oil affects the glucose and lipid metabolism in large yellow croaker Larimichthys crocea through the insulin-mediated PI3K/AKT signaling pathway Gu Z, et al,Comparative Biochemistry And Physiology B-Biochemistry & Molecular Biology,2019
- Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
- Growth response, body composition, plasma metabolites, digestive and antioxidant enzymes activities of Siberian sturgeon (Acipenser baerii, Brandt, 1869) fed different dietary protein and carbohydrate: lipid ratio Babaei S.et al,Aquaculture Research,2016
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