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中文名称:鱼热休克蛋白70(HSP-70)酶联免疫试剂盒
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货号:CSB-E16327Fh
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规格:96T/48T
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价格:¥3800/¥2500
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其他:
产品详情
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产品描述:鱼热休克蛋白70(HSP-70)酶联免疫试剂盒(CSB-E16327Fh)为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的HSP70含量。HSP70是重要的热休克蛋白,在细胞应激反应中起关键作用,广泛参与蛋白质折叠、转运和降解等过程。研究发现其异常表达与肿瘤、神经退行性疾病等多种疾病相关,抑制或激活HSP70功能正成为潜在治疗策略与研究热点。试剂盒检测范围为18.75 pg/mL-300 pg/mL,该产品为科研人员提供可靠工具,可应用于水产养殖健康监测、环境压力评估、鱼类生理机制研究等领域,为水生生物应激反应模型的建立及生态毒理学实验提供数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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别名:Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit
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缩写:HSP-70
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Uniprot No.:
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种属:Fish
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样本类型:serum, plasma, tissue homogenates
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检测范围:18.75 pg/mL-300 pg/mL
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灵敏度:18.75 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Signal Transduction
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 98 Range % 92-103 1:4 Average % 82 Range % 80-85 1:8 Average % 91 Range % 83-99 -
回收率:
The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-97 EDTA plasma (n=4) 94 91-98 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 300 0.153 0.144 0.149 150 0.255 0.242 0.249 75 0.519 0.533 0.526 37.5 1.103 1.134 1.119 18.75 2.363 2.270 2.317 -
数据处理:
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货期:3-5 working days
引用文献
- ACOUSTIC EFFECTS ON JUVENILE FISH IN BREEDING: CASE OF STUDY OF DICENTRARCHUS LABRAX M Mauro, JR Soriano, V Espinosa, P Poveda,/,/
- Determination of stress levels of Otolithoides biauritus (Cantor, 1849) in selected zones of Indian Sundarbans: Stress levels of Otolithoides biauritus in Sunderbans J Mitra, S Mondal,Indian Journal of Experimental Biology,2024
- Growth, Nutrient Deposition, Plasma Metabolites, and Innate Immunity are Associated with Feeding Rate in Juvenile Starry Flounder (Platichthys Stellatus) JH Cho,/,/
- TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
- Cadmium induced oxidative stress, endoplasmic reticulum (ER) stress and apoptosis with compensative responses towards the up-regulation of ribosome, protein processing in the ER, and protein export pathways in the liver of zebrafish W Hu,Aquatic toxicology,2023
- Effects of black soldier fly (Hermetia illucens) pre-pupae meal on the growth, stress, and immune responses of juvenile rainbow trout (Oncorhynchus mykiss) reared at different stocking densities JH Cho,Aquaculture Reports,2022
- Effect of temperature on food consumption, immune system, antioxidant enzymes, and heat shock protein 70 of Channa punctata (Bloch, 1793). SP Singh,fish physiology and biochemistry,2020
- Effect of Light Intensity on Survival, Growth and Physiology of Rohu, Labeo rohita (Cyprinidae) Fry Khan N A, et al,International Journal of Radiation Biology,2019
- INVESTIGATING THE ROLE OF HEAT SHOCK PROTEINS AND MELANOPHORES DURING STRESS CAUSED BY HIGH LIGHT INTENSITY IN INDIAN MAJOR CARP, CATLA CATLA N. A. Khan, et al,International Journal of Pharmaceutical Sciences and Research,2018
- Dietary supplementation of vitamin C: an effective measure to give protection against UV-B irradiation using fish as model organism Rina Chakrabarti, et al,Photochemical & Photobiological Sciences,2018
- Vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (Sparus aurata, Linnaeus 1758) Celi M. et al,Fish Physiol Biochem,2015
- Therapeutic effects of C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid (CDDO-Me; bardoxolone methyl) on radiation-induced lung inflammation and fibrosis in mice Wang YY. et al,Drug Des Devel Ther,2015
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