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Fish Glutathione Peroxidase (GSH-PX) ELISA kit

  • 中文名称:
    鱼谷胱甘肽过氧化物酶(GSH-PX)酶联免疫试剂盒
  • 货号:
    CSB-E15930Fh
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

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  • 产品描述:
    鱼谷胱甘肽过氧化物酶(GSH-PX)酶联免疫试剂盒(CSB-E15930Fh)为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的GSH-PX含量。GSH-PX(谷胱甘肽过氧化物酶)是一种重要的抗氧化酶,能够清除体内的活性氧,保护细胞免受氧化损伤。其研究机制主要涉及GSH-PX在肝脏疾病中的作用,如慢性肝炎、非酒精性脂肪性肝病、酒精性肝病、肝硬化和肝癌等,通过调节氧化应激和脂质过氧化反应,影响疾病的发生和发展。试剂盒检测范围为375 μU/mL-6000 μU/mL,适用于鱼类生理学研究、环境污染物对机体氧化损伤评估、水产饲料抗氧化添加剂效果分析等科研领域,为水产养殖健康管理、生态毒理学研究及营养调控提供数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    GSH-PX
  • 种属:
    Fish
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    375 μU/mL-6000 μU/mL
  • 灵敏度:
    187.5 μU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 91-99  
    1:200 Average % 103  
    Range % 99-107  
    1:400 Average % 98  
    Range % 93-103  
    1:800 Average % 92  
    Range % 87-97  
  • 回收率:
    The recovery of fish GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-104  
    EDTA plasma (n=4) 89 84-94  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μU/ml OD1 OD2 Average    
    6000 0.089 0.084 0.087    
    3000 0.246 0.265 0.256    
    1500 0.551 0.580 0.566    
    750 0.933 0.981 0.957    
    375 1.646 1.569 1.608    
    0 2.362 2.398 2.380    
  • 数据处理:
  • 货期:
    3-5 working days

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