Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

Fish Cytochrome P450 1A1(CYP1A1) ELISA kit

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中文名称：

鱼细胞色素P450 1A1(CYP1A1)酶联免疫试剂盒
货号：

CSB-EL006395FI
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

鱼细胞色素P450 1A1(CYP1A1)酶联免疫试剂盒（CSB-EL006395FI）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYP1A1含量。CYP1A1是一种主要的细胞色素P450酶，广泛研究其激活致癌化合物的能力。它通过芳香烃受体（AhR）介导的转录激活，在代谢环境致癌物和膳食化合物中发挥重要作用。CYP1A1在肺癌等癌症的发生发展中扮演关键角色，其研究机制涉及致癌物激活、基因多态性、以及与AhR等受体的相互作用。试剂盒检测范围为75 pg/mL-1200 pg/mL，适用于科研场景中评估鱼类暴露于环境毒素后的生理响应、污染物生物有效性研究以及生态毒理学模型的建立。为水生生态系统健康监测、污染物代谢机制研究及水产养殖安全评估提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

cyp1a1 ELISA kit; cyp1aCytochrome P450 1A1 ELISA kit; EC 1.14.14.1 ELISA kit; CYPIA1 ELISA kit
缩写：

CYP1A1
Uniprot No.：

Q6JZS3
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

75 pg/mL-1200 pg/mL
灵敏度：

75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	100
	Range %	92-107
1:4	Average %	85
	Range %	80-91
1:8	Average %	88
	Range %	83-96

回收率：

The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	88-95
EDTA plasma (n=4)	94	91-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1200	0.146	0.147	0.147
600	0.238	0.254	0.246
300	0.524	0.553	0.539
150	1.112	1.135	1.124
75	2.338	2.277	2.308

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Liver Structure and Function in Yellowtail Kingfish, Seriola lalandi, in Response to Reduced Fish Meal Diets BH Crowe, JO Harris, TJ McWhorter,Aquaculture, Fish and Fisheries,2024
Triklorfonun Gammarus pulex'te Bazı Enzimatik Antioksidanlara Etkisinin Değerlendirilmesi ŞT Yolcular,Ulusal Çevre Bilimleri Araştırma Dergisi,/
Dietary Artemisia arborescens Supplementation Effects on Growth, Oxidative Status, and Immunity of Gilthead Seabream (Sparus aurata L.) OP Tzortzatos,Animals,2024
Dimethoate ve Malathion Pestisitlerinin Dreissena polymorpha (Pallas, 1771) Üzerindeki Kombine Etkisinin Sitokrom P450 ve Asetilkolinesteraz Kullanılarak Belirlenmesi NC Yildirim,researchgate,2022
Toxicological effects of di-(2-ethylhexyl) phthalate in Gammarus pulex: a biochemical and histopathological assessment NC Yildirim,Environmental Science and Pollution Research,2021
The assessment of basic blue 41 textile dye decolorisation using the Fenton process: response surface methodology optimisation and toxicity evaluation M Tanyol,Chemistry and Ecology,2021
Evaluation of the efficiency of chlorpyrifos-ethyl remediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae using response of some biochemical biomarkers GO Erguven,Environmental Science and Pollution Research,2020
Congo Red Effects on Detoxification and Metabolic Enzymes in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) Durali Danabas, et al,Turkish Journal of Agriculture - Food Science and Technology,2019
Cytochrome P450 and Glutathione-S-Transferase Activity are Altered Following Environmentally Relevant Atrazine Exposures in Crayfish (Faxoniusvirilis) Awali S, et al,Bulletin of Environmental Contamination and Toxicology,2019
The usability of oxidative stress and detoxification biomarkers in Gammarus pulex for ecological risk assessment of textile dye methyl orange Nuran Cikcikoglu Yildirim, et al,Chemistry And Ecology,2019
Exposure Through Runoff and Ground Water Contamination Differentially Impact Behavior and Physiology of Crustaceans in Fluvial Systems Alexandra N. Steele.et al,ARCH ENVIRON CON TOX,2018
Biochemical responses of Gammarus pulex to malachite green solutions decolorized by Coriolus versicolor as a biosorbent under batch adsorption conditions Yildirim NC.et al,Ecotoxicol Environ Saf,2018

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功能：

Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
亚细胞定位：

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
蛋白家族：

Cytochrome P450 family
数据库链接：

STRING: 8090.ENSORLP00000018073

UniGene: Ola.6266

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.