Fish Cholecystokinin(CCK) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO鱼类胆囊收缩素/肠促胰酶肽（CCK）定量检测试剂盒（货号CSB-E13054Fh），基于竞争法ELISA原理开发，适用于检测血清、血浆及组织匀浆样本中的CCK浓度。CCK是一种具有重要生理功能的多肽激素，在鱼类体内参与调控消化酶分泌、胆囊收缩及摄食行为等过程。该试剂盒检测范围覆盖200至5000 pg/mL，通过预包被特异性抗体实现目标蛋白的定量分析，实验流程包含标准品梯度稀释、样本孵育、显色反应及酶标仪读数等标准化步骤。产品提供完整检测组件，包含预包被96孔板、标准品、检测抗体、显色底物及终止液等核心试剂，支持科研人员开展鱼类内分泌调控机制、消化生理功能及营养代谢相关研究。本试剂盒适用于实验室环境下的样本检测，可应用于水产养殖研究、鱼类生理学实验或环境毒理学评估等场景，为水生生物基础研究提供高灵敏度的检测工具。
缩写：

CCK
Uniprot No.：

D7S9W7
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

200 pg/mL-5000 pg/mL
灵敏度：

125 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-105
1:2	Average %	89
	Range %	84-96
1:4	Average %	96
	Range %	91-102
1:8	Average %	93
	Range %	86-105

回收率：

The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	85-91
EDTA plasma (n=4)	95	86-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.