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中文名称：鱼(鲑鱼)瘦素(LEP)酶联免疫试剂盒
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货号：CSB-EL012870FI
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规格：96T/48T
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价格：￥4200/￥3000
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其他：
产品详情
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产品描述：CUSABIO鱼（鲑鱼）瘦素（LEP）酶联免疫检测试剂盒（货号CSB-EL012870FI），基于竞争法原理实现定量分析，适用于鱼类血清、血浆样本中LEP蛋白的精准检测。瘦素作为调控能量代谢的关键信号分子，在鱼类摄食行为、脂肪储存及生殖发育等生理过程中发挥重要作用，其水平变化可反映机体代谢状态与环境适应机制。本试剂盒检测范围覆盖62.5-1000 pg/mL，采用预包被高特异性抗体与酶标物竞争结合的原理，通过标准曲线计算样本浓度，配套提供优化的反应板、标准品、检测抗体及显色底物，操作流程标准化，可在4.5小时内完成实验。该试剂盒适用于鱼类生理学、营养学及环境毒理学研究，可用于探究饵料成分、温度变化或污染物暴露对鱼类代谢调控的影响，为水产养殖优化和生态保护研究提供可靠工具，实验结果需结合其他生物学指标综合分析。所有组分均经过严格质控，确保批次稳定性和检测灵敏度，仅限科研用途。
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缩写：
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Uniprot No.：
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种属：Fish(Salmon)
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样本类型：serum, plasma
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检测范围：62.5 pg/mL-1000 pg/mL
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灵敏度：31.25 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Metabolism
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 91 Range % 86-98 1:40 Average % 97 Range % 91-102 1:80 Average % 84 Range % 80-87 1:160 Average % 90 Range % 83-96 -
回收率：
The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-96 EDTA plasma (n=4) 97 93-102 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1000 0.082 0.086 0.084 500 0.190 0.200 0.195 250 0.483 0.444 0.464 125 0.945 0.959 0.952 62.5 1.672 1.555 1.614 0 2.591 2.670 2.631 -
数据处理：
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货期：3-5 working days
引用文献
- Dietary functional palatability enhancer improved growth and appetite in largemouth bass (Micropterus salmoides) fed a reduced fish meal diet X Zhi, Y Gu, W Zhao, S Xie, Y Bao, S Morais, D Li,Aquaculture Reports,2025
- Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus) RE Ibrahim,/,2024
- Sequence identification and expression characterization of leptin in the spotted scat, Scatophagus argus WX Li,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
- The feedback regulation of carbohydrates intake on food intake and appetite in grass carp (Ctenopharyngodon idella) XC Yuan,Fish Physiol Biochem,2021
- Lipid Deposition and Mobilisation in Atlantic Salmon Adipocytes M Bou,international journal of molecular sciences,2020
- Comparative analysis of glucose metabolism responses of large yellow croaker Larimichthys crocea fed diet with fish oil and palm oil Wang Q,Fish Physiology and Biochemistry,2019
- High level of dietary soybean oil affects the glucose and lipid metabolism in large yellow croaker Larimichthys crocea through the insulin-mediated PI3K/AKT signaling pathway Gu Z, et al,Comparative Biochemistry And Physiology B-Biochemistry & Molecular Biology,2019
- Stress, cortisol, and feed intake in rainbow trout (Oncorhynchus mykiss) - Can you stomach the stress? Hoyland, Maren.et al,/,2018
- Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
- Physiological responses of Atlantic salmon (Salmo salar L.) fed very low (3%) fishmeal diets supplemented with feeding-modulating crystalline amino acid Katerina Kousoulaki.et al,Aquaculture,2017
- Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat Rong Yao, et al,PLoS One.,2015
- The Expression of Leptin, Estrogen Receptors, and Vitellogenin mRNAs in Migrating Female Chum Salmon, Oncorhynchus keta: The Effects of Hypo-osmotic Environmental Changes Young Jae Choi et al, Asian Australas. J. Anim. Sci.,2014
- Metabolism, health and fillet nutritional quality in Atlantic salmon (Salmo salar) fed diets containing n-3-rich microalgae Katerina Kousoulaki. et al,Journal of Nutritional Science,2015
产品评价
相关问答
■ 常见问题解答
Q:
https://www.cusabio.com/ELISA-Kit/Fish-Salmon-leptin-LEP-ELISA-kit-87315.html
Have you ever tested tissue sample?
A:
Thanks for your inquiry!We only tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test tissue samplet,we suggest do pre-testing first.Here is the processing way on tissue samples.Pls check for your reference:
Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was removed and assayed immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was removed and assayed immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Q:
Is this kit also compatible to test Leptin levels in Zebrafish?
A:
Thanks for your inquiry!We suggest you do a pretest if you want to test zebrafish.
Q:
Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please send the manual.
e. Please provide your reference data for fish plasma samples.
A:
Thanks for your inquiry.
CSB-EL012870FI
This is a competitive kit. There is only one antibody in the kit.
The plate is coated with mouse monoclonal antibody. ( Immunogen is CHO-derived recombinant protein.）
Standard is CHO-derived recombinant protein.
Pls find attached manual.
We tested salmon serum and plasma and some common freshwater fish before and the test value is about
125pg/ml under 1:100 dilution. The final concentration is about 12.5ng/ml.
Pls let me know if you have any further questions. Thank you.
CSB-EL012870FI
This is a competitive kit. There is only one antibody in the kit.
The plate is coated with mouse monoclonal antibody. ( Immunogen is CHO-derived recombinant protein.）
Standard is CHO-derived recombinant protein.
Pls find attached manual.
We tested salmon serum and plasma and some common freshwater fish before and the test value is about
125pg/ml under 1:100 dilution. The final concentration is about 12.5ng/ml.
Pls let me know if you have any further questions. Thank you.
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