Duck Interferon γ,IFN-γ ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本试剂盒（CSB-E08902Du）专用于鸭γ干扰素（IFN-γ）的定量检测，采用双抗体夹心酶联免疫吸附法（Sandwich ELISA）原理，可精准分析血清、血浆及组织匀浆样本中的IFN-γ含量。鸭IFN-γ作为一种重要的细胞因子，在宿主抗病毒免疫反应和Th1型免疫应答调控中发挥核心作用，其动态变化对研究禽类免疫机制具有重要价值。试剂盒检测灵敏度高，线性范围为15.6-1000 pg/mL，可覆盖低浓度基础值至高浓度炎症状态下的蛋白水平检测需求。实验操作标准化，提供预包被特异性抗体的96孔板及优化试剂体系，适用于科研场景中鸭类病毒感染模型研究、疫苗免疫效果评价、免疫调节分子通路探索等方向。本产品严格遵循科研用途设计，通过标准曲线实现样本靶标浓度的精准计算，为禽类免疫学研究提供高效可靠的技术支持。
别名：

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：

IFN-
Uniprot No.：

Q9YGB9
种属：

Duck
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of duck IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	98
	Range %	92-106
1:4	Average %	85
	Range %	81-86
1:8	Average %	88
	Range %	83-94

回收率：

The recovery of duck IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	103	101-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
亚细胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
数据库链接：

KEGG: apla:101790439