Dog β2-microglobulin,BMG/β2-MG ELISA Kit

产品详情

产品描述：

犬β2微球蛋白（BMG/β2-MG）酶联免疫试剂盒（货号：CSB-E13768c）采用双抗体夹心法定量检测犬血清、血浆或组织匀浆样本中的β2微球蛋白。β2-MG是主要组织相容性复合体（MHC-I）的轻链组分，广泛存在于有核细胞表面，参与免疫识别与抗原呈递，其水平异常与肾脏疾病、免疫系统紊乱及肿瘤发生密切相关。本试剂盒检测范围为0.78-50 μg/mL，灵敏度达0.195 μg/mL，通过预包被特异性捕获抗体与检测抗体联合作用，在3小时内完成目标蛋白的定量分析，适用于科研场景下犬类肾脏功能评估、免疫相关疾病机制研究或药物毒性实验中生物标志物的检测。试剂盒提供标准品、检测抗体及显色系统等完整组分，操作流程标准化，可稳定检测多种生物样本中β2-MG的动态变化，为动物实验提供可靠的数据支持。
别名：

B2M ELISA Kit; Beta-2-microglobulin ELISA Kit; Fragment ELISA Kit
缩写：

B2M
Uniprot No.：

P19341
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 μg/mL-50 μg/mL
灵敏度：

0.2 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	92
	Range %	85-100
1:4	Average %	95
	Range %	85-105
1:8	Average %	95
	Range %	88-102

回收率：

The recovery of dog β2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-99
EDTA plasma (n=4)	95	91-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
亚细胞定位：

Secreted.
数据库链接：

UniGene: Cfa.29179