Dog insulin-like growth factor binding protein 3 (IGFBP3)ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬胰岛素样生长因子结合蛋白3(IGFBP3)酶联免疫试剂盒（CSB-EL011097DO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGFBP3含量。IGFBP3是一种重要靶点。它参与胰岛素样生长因子（IGF）信号通路的调控，在细胞的生长、增殖、凋亡等过程中发挥作用。相关研究机制主要聚焦于其与IGF的结合，以及对下游信号传导途径的影响，为肿瘤等疾病的治疗提供新方向。试剂盒检测范围为3.12 ng/mL-200 ng/mL，本试剂盒适用于科研场景中犬类生长发育研究、代谢紊乱模型建立、内分泌调控机制探索等领域，例如通过检测血清IGFBP3水平评估生长激素轴功能状态，或分析营养干预对组织IGF信号通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IGFBP3
Uniprot No.：

F1PQ91
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IGFBP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	94
	Range %	91-99
1:40	Average %	96
	Range %	90-100
1:80	Average %	97
	Range %	95-100
1:160	Average %	95
	Range %	89-103

回收率：

The recovery of dog IGFBP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	96	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.