Dog growth hormone,GH ELISA Kit

Dog growth hormone,GH ELISA Kit

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中文名称：

犬生长激素(GH)酶联免疫试剂盒
货号：

CSB-E14962c
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

犬生长激素（GH）酶联免疫试剂盒（货号：CSB-E14962c）采用双抗体夹心法原理，专为定量检测犬血清、血浆及组织匀浆样本中的生长激素水平而设计。GH作为垂体前叶分泌的关键蛋白激素，在调控犬类生长发育、代谢平衡及细胞增殖中发挥重要作用，其水平变化常与生长异常或内分泌疾病相关。本试剂盒检测灵敏度高，线性范围为0.625 ng/mL至40 ng/mL，通过预包被高特异性抗体实现精准捕获目标蛋白，结合酶标抗体显色系统进行定量分析，实验全程无需复杂预处理，仅需100μL样本即可完成检测。针对组织样本，建议离心去除杂质后取上清液进行检测，以确保数据可靠性。该产品适用于动物生长机制研究、代谢调控分析、药物或营养干预实验等科研领域，为探究犬类生理病理过程中的GH动态变化提供稳定工具，实验结果需配合标准曲线计算浓度值，建议重复检测以提高数据准确性。
别名：

GH1 ELISA Kit; GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：

GH1
Uniprot No.：

P33711
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	98
	Range %	91-105
1:4	Average %	89
	Range %	84-95
1:8	Average %	99
	Range %	92-106

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.853

2.734

2.794

2.632

2.087

2.067

2.077

1.916

1.504

1.498

1.501

1.339

0.897

0.929

0.913

0.751

2.5

0.550

0.521

0.536

0.374

1.25

0.383

0.363

0.373

0.211

0.625

0.271

0.284

0.277

0.116

0.158

0.165

0.162

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Age-specific variations in hematological and biochemical parameters in middle-and large-sized of dogs SH Lee,J Vet Sci,2019

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能参考文献：
1. Progesterone alone has no direct stimulatory effect on GH transcription in mammary gland. PMID: 18262383
2. Study provides evidence that progesteron might increase autocrine Growth Hormone production which might directly stimulate local or systemic IGF-I secretion in canine mammary tumours. PMID: 18367392
亚细胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
数据库链接：

KEGG: cfa:403795

STRING: 9615.ENSCAFP00000018659

UniGene: Cfa.3656

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.