Dog adrenomedullin,ADM ELISA Kit

Dog adrenomedullin,ADM ELISA Kit

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中文名称：

犬肾上腺髓质素(ADM)酶联免疫试剂盒
货号：

CSB-E09836c
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO犬肾上腺髓质素（ADM）定量检测酶联免疫试剂盒，采用双抗体夹心法原理，适用于科研领域对犬血清、血浆、组织匀浆及细胞培养上清中ADM含量的精准分析。ADM是一种具有扩张血管、调节血压及参与炎症反应的多功能生物活性肽，广泛分布于肾上腺髓质、心血管及神经组织中，其表达水平与心血管疾病、感染性疾病等病理生理过程密切相关。该试剂盒灵敏度高，检测范围覆盖15.6-1000 pg/mL，可满足低浓度样本检测需求，实验操作便捷，提供标准品及预包被板条，适用于体外实验环境下探索ADM在犬类疾病模型中的调控机制、药物干预研究或基础生理功能解析，为动物医学及比较医学研究提供可靠工具。本产品仅限科学研究使用，需严格按照说明书进行样本前处理及实验操作。
别名：

ADM ELISA Kit; ADM [Cleaved into: Adrenomedullin ELISA Kit; AM); Proadrenomedullin N-20 terminal peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; PAMP ELISA Kit; ProAM-N20)] ELISA Kit
缩写：

ADM
Uniprot No.：

O77559
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-99
1:2	Average %	96
	Range %	91-104
1:4	Average %	98
	Range %	90-110
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of dog ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-104
EDTA plasma (n=4)	97	90-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.675	2.641	2.658	2.502
500	2.159	2.144	2.152	1.996
250	1.312	1.310	1.311	1.155
125	0.778	0.788	0.783	0.627
62.5	0.377	0.360	0.369	0.213
31.25	0.276	0.264	0.270	0.114
15.6	0.190	0.195	0.193	0.037
0	0.154	0.158	0.156

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

AM and PAMP are potent hypotensive and vasodilatator agents.
亚细胞定位：

Secreted.
蛋白家族：

Adrenomedullin family
数据库链接：

KEGG: cfa:403817

STRING: 9615.ENSCAFP00000011183

UniGene: Cfa.3681

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.