Dog Osteocalcin/bone gla protein,OT/BGP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬骨钙素/骨谷氨酸蛋白（OT/BGP）特异性定量检测试剂盒，采用双抗体夹心法原理开发，适用于检测犬血清、血浆及组织匀浆样本中的OT/BGP含量。骨钙素作为成骨细胞分泌的重要非胶原蛋白，在骨代谢调控、钙离子稳态维持中发挥关键作用，其水平变化常作为骨骼健康研究的生物标志物。试剂盒检测灵敏度达0.312 ng/mL，定量区间覆盖0.312-20 ng/mL，可精准检测生理及病理状态下样本中微量OT/BGP的浓度波动。该试剂盒包含预包被捕获抗体、生物素标记检测抗体及标准品等核心组分，操作流程经过严格验证，可在4.5小时内完成96孔板实验。科研人员可将其应用于犬类骨骼发育研究、骨质疏松模型构建、骨代谢相关药物筛选，以及营养干预对骨组织影响等基础研究领域，为犬科动物骨生物学研究提供可靠的数据支持。
别名：

BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
缩写：

BGLAP
Uniprot No.：

P81455
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	87-102
1:2	Average %	92
	Range %	89-98
1:4	Average %	89
	Range %	84-96
1:8	Average %	90
	Range %	86-99

回收率：

The recovery of dog OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-96
EDTA plasma (n=4)	102	95-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
亚细胞定位：

Secreted.
蛋白家族：

Osteocalcin/matrix Gla protein family
数据库链接：

STRING: 9615.ENSCAFP00000024773

UniGene: Cfa.3627