Dog N-terminal Pro atrial natriuretic peptide (NT-ProANP)ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬N端前心钠肽（NT-ProANP）酶联免疫检测试剂盒（货号：CSB-EQ027254DO），采用双抗体夹心法定量分析血清或血浆样本中的NT-ProANP蛋白。NT-ProANP是心钠肽前体裂解产生的生物标志物，主要反映心脏负荷状态及功能异常，在心血管调节中具有重要作用。试剂盒检测范围为0.312 ng/mL至20 ng/mL，通过预包被高特异性抗体实现精准定量，适用于低浓度样本检测，配套标准品提供可靠校准曲线。该产品可应用于犬类心血管疾病机制研究、心脏功能评估模型构建，以及药物干预或生理病理条件下心脏相关分子变化的动态监测，为动物实验中心脏健康研究提供高效工具。实验操作需严格按照说明书进行，仅限科研使用。
缩写：

NT-ProANP
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog NT-ProANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	91-105
1:2	Average %	97
	Range %	91-100
1:4	Average %	100
	Range %	92-106
1:8	Average %	95
	Range %	86-98

回收率：

The recovery of dog NT-ProANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	99	95-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.