Dog Myeloperoxidase (MPO) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬髓过氧化物酶（MPO）酶联免疫试剂盒（CSB-E12762c）是一款用于定量检测犬科生物样本中MPO含量的科研工具。MPO是中性粒细胞分泌的关键酶类，参与宿主先天免疫防御机制，其活性与炎症反应、氧化应激等病理过程密切相关，是研究感染性疾病、组织损伤修复和免疫调节的重要生物标志物。CUSABIO试剂盒采用夹心法原理，可精准检测31.25-2000 ng/mL浓度范围的MPO，适用于血清、血浆、细胞培养上清及组织匀浆等多种样本类型。试剂盒经过严格验证，具有高灵敏度和特异性，操作流程包含预包被板、标准品及检测抗体等核心组分，可通过标准曲线实现目标蛋白的精确定量。科研人员可将其应用于犬类炎症性疾病模型构建、药物抗炎效果评估、免疫调控机制研究等领域，为兽医病理学、比较医学及动物模型研究提供可靠的数据支持。该产品仅限科学研究使用，实验过程需严格遵循生物安全规范。
缩写：

MPO
Uniprot No.：

F1PQ52
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

31.25 ng/mL-2000 ng/mL
灵敏度：

7.81 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-94
1:2	Average %	101
	Range %	97-106
1:4	Average %	90
	Range %	87-95
1:8	Average %	91
	Range %	87-98

回收率：

The recovery of dog MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-95
EDTA plasma (n=4)	91	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.