Dog Matrix metalloproteinase 3,MMP-3 ELISA kit

Dog Matrix metalloproteinase 3,MMP-3 ELISA kit

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中文名称：

犬基质金属蛋白酶3(MMP-3)酶联免疫试剂盒
货号：

CSB-E13621c
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO犬基质金属蛋白酶3（MMP-3）定量检测试剂盒（货号：CSB-E13621c），采用双抗体夹心酶联免疫吸附法（Sandwich ELISA），可精准检测血清、血浆、细胞培养上清、尿液及组织匀浆等多种样本中MMP-3的浓度。MMP-3作为基质金属蛋白酶家族成员，在细胞外基质降解、炎症反应调控及组织重塑中发挥重要作用，其水平变化常与炎症性疾病、退行性关节病变等病理过程相关。试剂盒检测范围为1.56-100 ng/mL，灵敏度达1.56 ng/mL，适用于科研场景中犬类生物标志物研究，例如探索炎症相关疾病的分子机制、评估药物对组织修复的影响或建立特定病理模型。实验流程包含标准品梯度稀释与样本同步孵育，通过生物素标记检测抗体与链霉亲和素-HRP系统的级联放大实现信号检测，操作全程约需4.5小时。试剂盒包含预包被微孔板、标准品、检测抗体及显色底物等核心组分，支持科研人员高效开展犬类MMP-3表达水平的定量分析，为动物模型研究提供可靠工具。
别名：

MMP3Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit
缩写：

MMP3
Uniprot No.：

Q6Y4Q5
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	89
	Range %	85-96
1:4	Average %	93
	Range %	89-97
1:8	Average %	99
	Range %	92-105

回收率：

The recovery of dog MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	92	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.410	2.545	2.478	2.284
50	1.960	1.982	1.971	1.777
25	1.595	1.563	1.579	1.385
12.5	1.177	1.201	1.189	0.995
6.25	0.786	0.789	0.788	0.594
3.12	0.458	0.462	0.460	0.266
1.56	0.278	0.262	0.270	0.076
0	0.193	0.195	0.194

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Effects of SKCPT on Osteoarthritis in Beagle Meniscectomy and Cranial Cruciate Ligament Transection Models HM Kim,International journal of molecular sciences,2023
Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease Manuel R. Schmidli.et al,BMC VET RES,2018
Evaluation of Glucosamine and Snail Mucin on the Progression of Experimental Knee Osteoarthritis in Dogs Adetola R. Ajadi et al,International Journal of Morphology,2013

功能：

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
基因功能参考文献：
1. Findings suggested that increased TGF-beta3 and MMP-3 contribute to the pathogenesis of valvular degeneration associated with chronic mitral valve disease. PMID: 21281193
2. Both mRNA transcript levels and the activities of MMP-3 and MMP-7 are elevated in X-linked Alport syndrome (XLAS) kidneys from dogs. PMID: 15782307
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
数据库链接：

KEGG: cfa:403445

STRING: 9615.ENSCAFP00000022195

UniGene: Cfa.3447

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.