Dog Interleukin-33(IL33) ELISA kit

产品详情

产品描述：

犬白介素33（IL33）酶联免疫试剂盒（货号：CSB-EL011656DO）是一款基于夹心法原理设计的定量检测工具，适用于科研领域中对犬IL33蛋白表达水平的精准分析。IL33是免疫调节的重要分子，在Th2型免疫应答、组织炎症及宿主防御中发挥关键作用，其表达水平与多种病理生理过程密切相关。本试剂盒可检测血清、血浆及组织匀浆样本中的IL33蛋白，检测范围为23.44-1500 pg/mL，最低检测限为23.44 pg/mL，具有高灵敏度和特异性。通过优化的双抗体夹心体系，试剂盒能够稳定捕获目标蛋白并实现定量分析，适用于探索犬类疾病模型中的免疫调控机制、炎症相关研究或药物干预实验中的生物标志物检测。其操作流程兼容常规实验室条件，可为兽医基础研究、比较医学或实验动物模型开发提供可靠的数据支持，帮助科研人员深入解析IL33在犬类免疫系统中的功能及调控网络。
别名：

IL33Interleukin-33 ELISA Kit; IL-33 ELISA Kit; Protein DVS27 ELISA Kit
缩写：

IL33
Uniprot No.：

O97863
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

23.44 pg/mL-1500 pg/mL
灵敏度：

5.86 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-89
1:2	Average %	102
	Range %	97-107
1:4	Average %	89
	Range %	85-93
1:8	Average %	96
	Range %	93-90

回收率：

The recovery of dog IL33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-95
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.
亚细胞定位：

Nucleus. Chromosome. Cytoplasm. Cytoplasmic vesicle, secretory vesicle. Secreted.
蛋白家族：

IL-1 family
组织特异性：

Expressed in cultured umbilical artery smooth muscle cells after stimulation with IL1A and IL1B, and to a lesser extent with IFNG. Expressed in vasospastic cerebral arteries after subarachnoid hemorrhage.
数据库链接：

KEGG: cfa:403810

UniGene: Cfa.3672