Dog Interleukin 2,IL-2 ELISA Kit

产品详情

产品描述：

CUSABIO犬白介素2（Canine IL-2）酶联免疫检测试剂盒（货号CSB-E11258c），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中IL-2蛋白含量的精准分析。犬IL-2作为重要的免疫调节因子，在T淋巴细胞增殖、抗体生成及抗病毒免疫应答中发挥核心作用，其浓度变化可反映机体免疫状态。该试剂盒具有15.6 pg/mL至1000 pg/mL的宽泛线性检测范围，通过预包被高特异性抗体实现目标蛋白捕获，配合HRP标记检测抗体形成夹心复合物，显色后通过标准曲线进行定量计算。实验全程约需4.5小时，包含标准品、检测抗体及显色底物等完整组分，适用于兽医科研领域对犬类免疫相关疾病的机制研究，如感染性疾病模型建立、疫苗免疫效果评估、炎症反应动态监测等实验场景。组织样本前处理建议按10%比例制备匀浆液，离心后取上清检测以确保数据准确性。
别名：

IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写：

IL2
Uniprot No.：

Q29416
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	95
	Range %	92-98
1:4	Average %	86
	Range %	83-90
1:8	Average %	97
	Range %	94-100

回收率：

The recovery of dog IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	82-90
EDTA plasma (n=4)	105	101-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能参考文献：
1. immunoreactivities of inflammatory cytokines, such as interleukin (IL)-2, its receptor (IL-2R), IL-4 and its receptor (IL-4R) in the cervical and lumbar spinal cord of young adult and aged beagle dogs, were compared. PMID: 23605681
2. In dogs with leishmaniasis, there was high expression of genes encoding IL-2 in lymph nodes. PMID: 21511273
亚细胞定位：

Secreted.
蛋白家族：

IL-2 family
数据库链接：

KEGG: cfa:403989

STRING: 9615.ENSCAFP00000005941

UniGene: Cfa.7