Dog Interleukin 1β (IL- 1β) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬白介素1β（IL-1β）酶联免疫检测试剂盒（货号：CSB-E13836c），基于双抗体夹心法原理实现定量分析，适用于检测血清、血浆、细胞培养上清液及组织匀浆样本中IL-1β的含量。IL-1β是参与犬类先天免疫和炎症反应的关键细胞因子，在感染、创伤或自身免疫性疾病中显著表达，其动态监测对研究犬类炎症机制及免疫调控具有重要意义。试剂盒检测灵敏度为7.8 pg/mL，线性范围为7.8-500 pg/mL，可精准捕捉样本中低浓度IL-1β的变化。实验操作采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立标曲，配合显色反应实现目标蛋白的定量分析。该产品适用于科研领域，如探究犬类模型中的炎症性疾病病理过程、评估免疫调节剂或抗炎药物的干预效果，以及动物疫苗研发中的免疫应答研究，为兽医学和比较医学领域的实验提供可靠工具。
别名：

IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写：

IL1B
Uniprot No.：

Q28292
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	83-100
1:2	Average %	94
	Range %	91-99
1:4	Average %	95
	Range %	90-105
1:8	Average %	94
	Range %	86-100

回收率：

The recovery of dog IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	97	90-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
亚细胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
数据库链接：

STRING: 9615.ENSCAFP00000010762

UniGene: Cfa.33592