Dog Interleukin 18,IL-18 ELISA KIT

Dog Interleukin 18,IL-18 ELISA KIT

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

犬白介素18(IL-18)酶联免疫试剂盒
货号：

CSB-E09848c
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

CUSABIO犬白介素18（IL-18）酶联免疫检测试剂盒（货号：CSB-E09848c），采用双抗体夹心法定量检测犬血清、血浆、细胞培养上清及组织匀浆中的IL-18蛋白浓度。IL-18作为促炎细胞因子，在先天免疫应答、感染性疾病及炎症调控中发挥重要作用，其动态监测可为犬类免疫机制研究、疾病模型建立及药物疗效评估提供关键数据支持。试剂盒检测灵敏度高，线性范围覆盖12.5-800 pg/mL，可精准捕捉样本中IL-18的微量表达变化。实验操作采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统显色，实现目标蛋白的稳定定量分析。适用于科研领域对犬类炎症性疾病（如败血症、自身免疫病）、肿瘤免疫微环境或疫苗免疫效果的研究，支持多类型样本同步检测，为动物实验中的免疫指标分析提供标准化工具。试剂盒包含标准品、检测抗体及全套实验缓冲液，用户仅需自备酶标仪即可完成检测，实验流程优化后可在4.5小时内获得可靠结果。
别名：

IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：

IL18
Uniprot No.：

Q9XSR0
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-109
1:2	Average %	97
	Range %	91-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of dog IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	80-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.675	2.552	2.614	2.439
400	2.065	2.076	2.071	1.896
200	1.372	1.424	1.398	1.223
100	0.726	0.739	0.733	0.558
50	0.471	0.504	0.488	0.313
25	0.380	0.359	0.370	0.195
12.5	0.271	0.259	0.265	0.090
0	0.173	0.177	0.175

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: cfa:403796

STRING: 9615.ENSCAFP00000038185

UniGene: Cfa.40

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.