Dog Interleukin-13(IL13) ELISA kit

中文名称：

犬白介素13(IL13)酶联免疫试剂盒
货号：

CSB-EL011590DO
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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犬白介素13（IL13）酶联免疫试剂盒（货号：CSB-EL011590DO）采用双抗体夹心ELISA技术，可定量检测犬血清、血浆及组织匀浆样本中的IL13蛋白，检测灵敏度为12.5 pg/mL，线性范围为12.5-800 pg/mL，适用于科研领域对犬类免疫调节机制的研究。IL13是一种由Th2细胞分泌的多效性细胞因子，在过敏反应、抗寄生虫免疫及炎症性疾病中发挥重要作用，其表达水平与组织纤维化、哮喘等病理过程密切相关。本试剂盒通过预包被高特异性捕获抗体与标记检测抗体的协同作用，确保检测的高灵敏度和准确性，操作流程包含标准品稀释、样本孵育、显色及读数步骤，实验全程约需4.5小时。科研人员可将其应用于犬类过敏性疾病模型构建、寄生虫感染后免疫应答评估、药物干预效果分析等研究，为探索犬类Th2型免疫反应提供可靠的工具支持。试剂盒兼容常规酶标仪检测，建议实验前通过预实验优化样本稀释比例以确保数据有效性。
别名：

IL13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit
缩写：

IL13
Uniprot No.：

Q9N0W9
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-102
1:2	Average %	92
	Range %	88-96
1:4	Average %	90
	Range %	86-94
1:8	Average %	87
	Range %	82-91

回收率：

The recovery of dog IL13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-112
EDTA plasma (n=4)	85	80-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.315	2.467	2.391	2.263
400	2.049	2.158	2.104	1.976
200	1.720	1.680	1.700	1.572
100	1.193	1.124	1.159	1.031
50	0.782	0.796	0.789	0.661
25	0.507	0.475	0.491	0.363
12.5	0.328	0.309	0.319	0.191
0	0.124	0.131	0.128

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
亚细胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
数据库链接：

KEGG: cfa:442990

STRING: 9615.ENSCAFP00000001250

UniGene: Cfa.3566

Dog Interleukin-13(IL13) ELISA kit

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