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  4. Dog Interleukin 10,IL-10 ELISA Kit

Dog Interleukin 10,IL-10 ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    犬白介素10(IL-10)酶联免疫试剂盒
  • 货号：
    CSB-E11261c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    犬白介素10（IL-10）酶联免疫试剂盒（货号：CSB-E11261c）是一款基于双抗体夹心法原理的定量检测工具，专为科研领域设计，可精准检测犬血清、血浆及组织匀浆样本中的IL-10蛋白浓度。IL-10是一种关键的抗炎细胞因子，在调节免疫应答、抑制炎症反应及维持免疫稳态中发挥重要作用，其水平变化与多种犬类免疫相关疾病的研究密切相关。本试剂盒检测灵敏度高，线性范围为1.56 pg/mL至100 pg/mL，可覆盖生理及病理状态下IL-10的浓度变化需求。试剂盒采用预包被特异性抗体的酶标板，通过标准化的孵育、洗涤和显色流程，确保实验结果的重复性与准确性。适用于犬类免疫机制研究、炎症性疾病模型评估、疫苗或药物干预效果分析等科研场景，为探索IL-10在感染、肿瘤或自身免疫性疾病中的调控机制提供可靠数据支持。操作流程简便，兼容常规实验室设备，满足高通量样本检测需求。
  • 别名：
    IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 缩写：
    IL10
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %92-108
    1:2Average %97
    Range %91-105
    1:4Average %89
    Range %84-96
    1:8Average %92
    Range %87-98
  • 回收率：
    The recovery of dog IL-10spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-95
    EDTA plasma (n=4)9287-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.954 2.967 2.961 2.853
    502.388 2.398 2.393 2.285
    251.758 1.786 1.772 1.664
    12.51.035 1.109 1.072 0.964
    6.250.502 0.546 0.524 0.416
    3.120.271 0.267 0.269 0.161
    1.560.184 0.196 0.190 0.082
    00.112 0.103 0.108 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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IL10 Proteins

相关问答

■ 常见问题解答
Q:

If using this kit it is necessary to perform a a blood clotting time of 2 hours or overnight at 4 ° C and 15 minutes of centrifugation, and if so what is the reason for this procedure.

A:
Thanks for your inquiry.
It is the sample preparation method for serum indeed.
It is fine to do as the you mentioned for serum. Here also attached our manual for your checking.
The sample preparation method is through our our experimental experience and get it to prepare relatively large amounts of serum samples with relatively simple components.Pls let me know if you have any further questions. Thank you.
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靶点详情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 基因功能参考文献：
    1. seven new genetic variations of the IL10 gene were identified. PMID: 24487092
    2. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
    3. serum and tumor levels of IL-8 and IL-10 in dogs bearing benign and malignant mammary tumors, including dogs with inflammatory mammary cancer, for a better understanding of this disease PMID: 23351639
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 数据库链接：

    KEGG: cfa:403628

    STRING: 9615.ENSCAFP00000016812

    UniGene: Cfa.38

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |