Dog Interferon γ,IFN-γ ELISA Kit

Dog Interferon γ,IFN-γ ELISA Kit

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中文名称：

犬γ干扰素(IFN-γ)酶联免疫试剂盒
货号：

CSB-E11262c
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO犬γ干扰素（IFN-γ）酶联免疫吸附检测试剂盒（货号：CSB-E11262c），采用双抗体夹心检测原理，可对血清、血浆及组织匀浆样本中的犬IFN-γ进行定量分析，检测灵敏度达3.12 pg/mL，线性范围为3.12-200 pg/mL。犬IFN-γ是由活化的T淋巴细胞和自然杀伤细胞分泌的关键细胞因子，在抗病毒免疫、肿瘤免疫监视及巨噬细胞活化中发挥重要作用。本试剂盒通过特异性抗体对目标蛋白进行捕获与检测，适用于科研场景中犬类免疫相关研究，例如评估感染性疾病模型中的免疫应答强度、监测疫苗免疫效果、探究炎症性疾病机制或分析免疫调节药物的作用效果。实验操作兼容常规酶标仪检测，提供标准品及预包被板条，支持多样本类型的高通量分析，为犬类免疫学、兽医学研究及生物制剂开发提供可靠工具。
别名：

IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：

IFNG
Uniprot No.：

P42161
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-104
1:2	Average %	96
	Range %	92-102
1:4	Average %	95
	Range %	89-102
1:8	Average %	97
	Range %	90-101

回收率：

The recovery of dog IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-100
EDTA plasma (n=4)	96	89-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.885	1.853	1.869	1.766
100	1.226	1.308	1.267	1.164
50	0.891	0.924	0.908	0.805
25	0.636	0.686	0.661	0.558
12.5	0.407	0.425	0.416	0.313
6.25	0.310	0.318	0.314	0.211
3.12	0.248	0.252	0.250	0.147
0	0.102	0.104	0.103

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
基因功能参考文献：
1. IL-17A gene transcription level was positively correlated with mRNA expression for iNOS and IFN-gamma PMID: 26590047
2. In dogs with leishmaniasis, there was high expression of interferon gamma in lymph nodes. PMID: 21511273
3. These findings suggest that polyunsaturated fatty acids are able to influence proliferation of peripheral blood mononuclear cells in healthy and atopic dogs but do not seem to influence cytokine transcription. PMID: 20187917
亚细胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
组织特异性：

Released primarily from activated T lymphocytes.
数据库链接：

KEGG: cfa:403801

STRING: 9615.ENSCAFP00000000588

UniGene: Cfa.3900

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.