Dog Erythropoietin,EPO ELISA Kit

产品详情

产品描述：

犬红细胞生成素（EPO）酶联免疫试剂盒（货号：CSB-E11303c）采用双抗体夹心法定量检测犬血清、血浆或组织匀浆样本中的EPO水平，检测灵敏度为1.56 mIU/mL，线性范围覆盖1.56-100 mIU/mL。EPO是一种由肾脏分泌的糖蛋白激素，在红细胞生成调控中起核心作用，其表达水平与贫血、慢性肾病及造血功能异常密切相关。本试剂盒通过高特异性抗体实现精准检测，适用于科研场景中犬类贫血机制研究、肾脏功能与造血系统关联性分析、药物干预效果评价或疾病模型（如肾衰竭、肿瘤性贫血）的EPO动态监测。实验操作简便，兼容常规酶标仪分析，配套试剂提供完整检测体系，支持多样本类型的高通量检测需求，为动物实验研究提供可靠的数据支持。
别名：

EPOErythropoietin ELISA kit
缩写：

EPO
Uniprot No.：

P33707
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 mIU/mL-100 mIU/mL
灵敏度：

0.39 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-105
1:2	Average %	89
	Range %	80-97
1:4	Average %	93
	Range %	86-100
1:8	Average %	95
	Range %	88-100

回收率：

The recovery of dog EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	83-102
EDTA plasma (n=4)	95	85-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
基因功能参考文献：
1. Developmental and compensatory lung growth involve paracrine erythropoietin signaling with parallel upregulation but differential processing of the erythropoietin receptor. PMID: 15286000
2. apical secretion of EPO involves a cholesterol-dependent mechanism probably not involving lipid rafts PMID: 15916748
亚细胞定位：

Secreted.
蛋白家族：

EPO/TPO family
组织特异性：

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
数据库链接：

KEGG: cfa:404002

STRING: 9615.ENSCAFP00000038507

UniGene: Cfa.3871