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  4. Dog Endothelin 1,ET-1 ELISA Kit

Dog Endothelin 1,ET-1 ELISA Kit

说明书下载
  • 中文名称：
    犬内皮素1(ET-1)酶联免疫试剂盒
  • 货号：
    CSB-E07002c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO犬内皮素1（Endothelin-1, ET-1）酶联免疫检测试剂盒（货号：CSB-E07002c），采用夹心法原理实现定量检测，适用于科研领域对犬血清、血浆及组织匀浆样本中ET-1含量的精准分析。ET-1是一种由血管内皮细胞分泌的强效血管收缩肽，在调节心血管功能、炎症反应及组织损伤修复中发挥关键作用，其水平变化常与犬类心血管疾病、呼吸系统疾病及代谢紊乱等病理过程相关。本试剂盒检测范围为3.12-200 pg/mL，灵敏度高，可稳定检测低浓度样本。实验操作流程标准化，通过预包被特异性抗体的微孔板捕获目标蛋白，结合标记抗体进行显色反应，适用于实验室对犬模型在基础研究、药物作用机制探索或疾病模型构建中的ET-1动态监测。试剂盒包含标准品、检测抗体及必要反应试剂，兼容常规酶标仪读数，为科研人员提供高效、可靠的检测方案。
  • 别名：
    EDN1Endothelin-1 ELISA kit; ET-1 ELISA kit; Preproendothelin-1 ELISA kit; PPET1) [Cleaved into: Big endothelin-1] ELISA kit
  • 缩写：
    EDN1
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %85-96
    1:2Average %94
    Range %90-99
    1:4Average %89
    Range %81-96
    1:8Average %100
    Range %91-108
  • 回收率：
    The recovery of dog ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8782-96
    EDTA plasma (n=4)9782-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.954 2.989 2.972 2.861
    1002.388 2.398 2.393 2.282
    501.758 1.789 1.774 1.663
    251.035 1.069 1.052 0.941
    12.50.502 0.543 0.523 0.412
    6.250.271 0.268 0.270 0.159
    3.120.184 0.169 0.177 0.066
    00.112 0.110 0.111 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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EDN1 Proteins

靶点详情

  • 功能：
    Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
  • 基因功能参考文献：
    1. These findings suggest that elevated serum big ET-1 could be used as a diagnostic marker for canine hemangiosarcoma . PMID: 25920760
    2. This study showed that canine osteosarcoma cells express endothelin-1 and functional endothelin A receptors; increases in bone alkaline phosphatase activity are associated with osteoblastic OS lesions. PMID: 26426813
    3. There was a progressive increase in ET-1 and ETAR expression from benign tumour to grade 1 and grade 2 malignant mammary tumours with a decrease of expression in grade 3 carcinomas. Co-localization of ET-1 and ETAR was observed in benign mammary tumours. PMID: 25816929
    4. These findings point to the potential of serum big ET-1 as a clinical marker for cardiopulmonary and neoplastic diseases in dogs. PMID: 24447631
    5. Renal denervation substantially decreased BP and also significantly decreased the plasma levels of renin-angiotensin system and ET-1. PMID: 24496516
    6. These results showed that endothelin-1 and endothelin receptor A are overexpressed in canine ovarian tumours, suggesting a potential role of these two molecules in canine ovarian carcinogenesis. PMID: 20210880
    7. ET-1 production in macrophage cell line DH82 was increased in response to stimulation with intact and viable gram-positive and gram-negative bacteria. PMID: 20207425
    8. The results showed that there was an interaction between ET-1 and NO in a VILI (ventilator-induced lung injury )model and changing the balance of ET-1 and NO levels might contribute to the pathophysiologic process of VILI. PMID: 20497708
    9. Report endothelin levels in experimental diabetes combined with cardiac hypertrophy. PMID: 15838277
    10. Report changes in endothelin-1 gene expression during myocardial ischemia and reperfusion. PMID: 15838278
    11. Intrapericardial adenine nucleosides induce elevation of endothelin-1 concentration in the pericardial space of the dog heart. PMID: 15838286
    12. The results confirm that intrapericardial catecholamines exert significant hemodynamic effects and modulate ET-1 and adenosine release from the heart. PMID: 15838288
    13. Report changes in endothelin-1/big endothelin-1 levels and action potential duration during myocardial ischemia-reperfusion in dogs with and without ventricular fibrillation. PMID: 15838324
    14. although ET(A) receptor-signaling is sensitized by induction of the metabolic syndrome, endothelin-mediated coronary vasoconstriction does not significantly contribute to coronary dysfunction at this early stage of prediabetes PMID: 16627363
    15. These results may potentially explain resting perfusion abnormalities or heterogeneity by clinical PET that may persist or only partially improve after adenosine stress perfusion imaging in the absence of myocardial scar and flow-limiting stenosis. PMID: 16951040
    16. Alterations in blood levels of endothelin-1, atrial natriuretic peptide, aldosterone, and norepinephrine in dogs with and without dilated cardiomyopathy are reported. PMID: 17338155
    17. Early pulmonary hypertension in heart failure is characterized by altered vasoreactivity and increased ET-1 and VEGF2 signalling. PMID: 17993509
    18. peripheral NO and ET-1 release modulates the cardiac contractility through myocardial ET-A receptor activation and mast cell degranulation. PMID: 18788466
    19. The use of Plasma atrial natriuretic peptide (proANP 31-67), B-type natriuretic peptide (Nt-proBNP) and endothelin-1 (ET-1) concentrations as disease markers in dogs with valvular heart disease is reported. PMID: 19585255

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Endothelin/sarafotoxin family
  • 数据库链接：

    KEGG: cfa:403424

    STRING: 9615.ENSCAFP00000014389

    UniGene: Cfa.125

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |