Dog Apolipoprotein A-I(APOA1) ELISA kit

Dog Apolipoprotein A-I(APOA1) ELISA kit

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中文名称：

犬载脂蛋白A1(Apo-A1)酶联免疫试剂盒
货号：

CSB-EL001913DO
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO犬载脂蛋白A1（Apo-A1）酶联免疫试剂盒（货号：CSB-EL001913DO），采用竞争法原理实现定量检测，适用于犬血清、血浆及组织匀浆样本中Apo-A1含量的精准分析。Apo-A1是高密度脂蛋白（HDL）的主要结构蛋白，在脂质代谢调控和心血管健康研究中具有重要科学价值，其水平变化常作为评估脂质代谢异常及相关疾病的潜在生物标志物。本试剂盒检测范围为28.12-1800 ng/mL，可覆盖多数生理及实验条件下样本的检测需求。该产品基于高特异性抗体开发，操作流程标准化，适用于科研领域中对犬类脂代谢机制、心血管疾病模型构建、营养干预效果评估等研究，尤其在探究HDL功能与疾病相关性、药物或膳食成分对脂代谢影响的实验中具有广泛应用。组织匀浆兼容性设计还可支持特定器官或病理组织中Apo-A1的分布研究，为深入探索犬类脂质代谢的分子机制提供可靠工具。
别名：

APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
缩写：

APOA1
Uniprot No.：

P02648
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

28.12 ng/mL-1800 ng/mL
灵敏度：

7 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	93
	Range %	89-103
1:2000	Average %	89
	Range %	85-95
1:4000	Average %	95
	Range %	84-103
1:8000	Average %	93
	Range %	83-98

回收率：

The recovery of dog APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	92-95
EDTA plasma (n=4)	95	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1800	0.142	0.145	0.144
900	0.215	0.229	0.222
450	0.382	0.367	0.375
225	0.551	0.558	0.555
112.5	0.973	0.990	0.982
56.25	1.697	1.623	1.660
28.12	2.287	2.328	2.308
0	2.654	2.761	2.708

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
亚细胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
组织特异性：

Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
数据库链接：

STRING: 9615.ENSCAFP00000019630

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.