Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

Deer Insulin-like growth factors 1,IGF-1 ELISA Kit

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中文名称：

鹿胰岛素样生长因子1(IGF-1)酶联免疫试剂盒
货号：

CSB-E12644De
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO鹿胰岛素样生长因子1（IGF-1）酶联免疫检测试剂盒（货号：CSB-E12644De），采用竞争法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中的IGF-1水平，检测灵敏度范围为1-200 ng/mL。IGF-1是一种多肽类生长调节因子，在鹿的生长发育、细胞增殖分化及代谢调控中发挥重要作用，其浓度变化与动物生长性能、营养状态及生理周期密切相关。该试剂盒通过特异性抗体与样本中的IGF-1结合，结合竞争性反应体系实现精准定量，实验流程包含标准品梯度稀释、样本预处理及显色测定等标准化步骤，可在4-6小时内完成检测。科研人员可将其应用于鹿科动物生长发育研究、营养干预实验、繁殖生物学分析以及代谢相关疾病模型的机制探索，例如评估不同饲养条件下IGF-1的动态变化，或探究其在组织再生修复中的作用。试剂盒包含预包被板、检测抗体、标准品及全套实验试剂，支持96孔板规格批量检测，提供标准曲线建立与数据计算方案，满足实验室高效、稳定的科研需求。
缩写：

IGF-1
Uniprot No.：

H9BDM1
种属：

Deer
样本类型：

serum, plasma, tissue homogenates
检测范围：

1 ng/mL-200 ng/mL
灵敏度：

0.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	95
	Range %	89-104
1:4	Average %	99
	Range %	87-107
1:8	Average %	96
	Range %	90-102

回收率：

The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.175	0.174	0.175
50	0.333	0.316	0.325
16	0.622	0.640	0.631
4	1.040	1.048	1.044
1	1.416	1.389	1.403
0	2.577	2.633	2.605

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

SWATH-MS Quantitative Proteomic Analysis of Deer Antler from Two Regenerating and Mineralizing Sections M López-Pedrouso,Biology,2021
Reproductive responses to sexually active buck of does treated with melatonin when body weight/body condition is increasing or decreasing Gallego-Calvo, L.et al, Animal Reproduction Science,2018
Detection and quantitation of insulin-like growth factor-1 in velvet antler by an enzyme-linked immunosorbent assay /,Consumer Electronics, Communications and Networks (CECNet),2011

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.