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  4. Chicken growth hormone,GH ELISA KIT

Chicken growth hormone,GH ELISA KIT

文献引用 (9) 说明书下载
  • 中文名称：
    鸡生长激素(GH)酶联免疫试剂盒
  • 货号：
    CSB-E09866Ch
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO鸡生长激素（GH）酶联免疫检测试剂盒（货号CSB-E09866Ch），采用竞争法原理实现定量分析，适用于检测鸡血清、血浆及组织匀浆样本中GH的浓度水平，其检测范围覆盖625至10000 pg/mL的宽线性区间。鸡生长激素是由垂体前叶分泌的多肽类激素，在调节生长发育、蛋白质合成及能量代谢等生理过程中发挥关键作用，其表达水平与家禽生长性能密切相关。本试剂盒通过高特异性抗体实现靶标精准捕获，配套标准品提供稳定校准曲线，可满足科研场景下对GH的定量需求，例如探究家禽生长发育调控机制、评估营养干预对激素分泌的影响、建立疾病相关代谢模型等研究。该试剂盒操作流程标准化，兼容常规酶标仪检测，为农业科研领域研究家禽生理调控、品种选育及饲养管理优化提供可靠工具。
  • 别名：
    GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 缩写：
    GH
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    625 pg/mL-10000 pg/mL
  • 灵敏度：
    625 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Developmental Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-98  
    1:2 Average % 99  
    Range % 92-105  
    1:4 Average % 85  
    Range % 80-89  
    1:8 Average % 90  
    Range % 83-98  
  • 回收率：
    The recovery of chicken GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 88-96  
    EDTA plasma (n=4) 94 91-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    10000 0.154 0.145 0.150    
    5000 0.261 0.244 0.253    
    2500 0.538 0.534 0.536    
    1250 1.113 1.139 1.126    
    625 2.356 2.278 2.317    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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GH Proteins

靶点详情

  • 功能：
    Growth hormone plays an important role in growth control.
  • 基因功能参考文献：
    1. This is the first study to demonstrate that single nucleotide polymorphisms in GHSR can increase appetite, growth traits, expression and level of GHRL, suggesting a hunger signal role for endogenous GHRL. PMID: 27591070
    2. There is a negative glucocorticoid response-like element located in the +591/+609 region within the first intron of chicken growth hormone (cGH), which is essential for the down-regulation of cGH expression. PMID: 27834851
    3. It is shown that in all examined chicken populations the growth hormone gene is polymorphic. PMID: 26841491
    4. The molecular mechanisms underlying glucocorticoid recruitment of somatotrophs during embryogenesis were studied; these provide insight into glucocorticoid-induced developmental changes in other tissues and cell types. PMID: 25560830
    5. T185G growth hormone polymorphism was significantly associated with the egg production traits in chickens. PMID: 25062422
    6. GHRH stimulates testicular GH secretion in an autocrine or paracrine manner. PMID: 24508498
    7. The results indicate that high frequency of I, C and A alleles corresponds to the egg-laying chicken lines. PMID: 23285753
    8. A 1727-bp fragment of 5'-flanking region of chicken growth hormone (cGH) gene has been cloned and sequenced. PMID: 15229358
    9. small chicken GH expression and translation occurs in ocular tissues of chick embryos, in which its localization in the neural retina and the optic nerve head is distinct from that of the full-length protein PMID: 17008400
    10. The identification of 3 restriction enzyme polymorphic sites were detected in the growth hormone gene is reported. PMID: 17495076
    11. Results showed that there was no significant difference between dwarf and normal chickens in GH expression. PMID: 17681929
    12. results demonstrate presence of growth hormone and its receptors in the ovarian stroma with cortical follicles & in the wall of small and large follicles of the ovary during sexual maturation PMID: 18335240
    13. Positive somatotroph responses to corticosterone (CORT) are lost during chicken embryonic development and that glucocorticoid receptors are the primary regulator of CORT-induced growth hormone secretion. PMID: 19157766

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    KEGG: gga:378781

    STRING: 9031.ENSGALP00000000327

    UniGene: Gga.48744

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |