Chicken Leptin,LEP ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO鸡瘦素（LEP）酶联免疫检测试剂盒（货号：CSB-E14082C），采用双抗体夹心法原理实现定量检测，适用于科研领域中对鸡血清、血浆及组织匀浆样本中LEP蛋白含量的精准分析。瘦素作为脂肪组织分泌的关键代谢调节因子，在能量平衡、食欲调控及生殖功能中发挥重要作用，其水平变化常与营养状态及代谢性疾病相关研究密切相关。该试剂盒具备0.312 ng/mL至20 ng/mL的宽线性检测范围，可灵敏捕捉样本中LEP的浓度梯度变化，操作流程严格遵循标准ELISA技术规范。科研人员可通过本试剂盒开展家禽生长调控机制、脂肪代谢通路、繁殖生理学以及营养干预等基础研究，为禽类健康管理及遗传育种提供实验依据。试剂盒包含预包被特异性抗体的96孔板、标准品、检测抗体及显色系统，支持用户高效完成样本检测与数据分析。
别名：

LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
缩写：

LEP
Uniprot No.：

O42164
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-101
1:2	Average %	95
	Range %	85-104
1:4	Average %	91
	Range %	87-100
1:8	Average %	93
	Range %	89-106

回收率：

The recovery of chicken LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-107
EDTA plasma (n=4)	95	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways.
亚细胞定位：

Secreted.
蛋白家族：

Leptin family
组织特异性：

Not exclusively localized in adipose tissue but is also expressed in liver.