Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Chicken Interleukin 8(IL-8) ELISA Kit

Chicken Interleukin 8(IL-8) ELISA Kit

文献引用 (7) 说明书下载
  • 中文名称：
    鸡白介素8(IL-8)酶联免疫试剂盒
  • 货号：
    CSB-E14191C
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO鸡白介素8（IL-8）酶联免疫检测试剂盒（货号CSB-E14191C），适用于血清、血浆及组织匀浆样本中IL-8含量的精准定量分析。IL-8是禽类重要的趋化因子，在机体免疫应答中发挥关键作用，参与炎症反应调控和中性粒细胞趋化过程，其水平变化可反映感染性疾病或免疫应激状态。试剂盒采用竞争法检测原理，检测灵敏度达1.56 pg/mL，线性范围为1.56-400 pg/mL，可实现微量样本中IL-8的稳定检测。该产品广泛用于家禽免疫机制研究、疫苗效力评估、病原感染模型建立等科研领域，特别适用于鸡传染性支气管炎、新城疫等疾病模型中炎症因子的动态监测，以及免疫调节剂或饲料添加剂对免疫系统影响的量化评估。实验支持多种样本类型检测，组织匀浆检测功能为深入解析局部组织免疫反应提供技术保障。本产品严格遵循科研检测标准，实验数据稳定可靠，为禽类免疫学研究提供标准化工具。
  • 别名：
    CXCL8 ELISA Kit; CEF4 ELISA Kit; EMF1 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; 9E3 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Embryo fibroblast protein 1 ELISA Kit; EMF-1 ELISA Kit
  • 缩写：
    CXCL8
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-400 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 102  
    Range % 97-106  
    1:2 Average % 88  
    Range % 84-93  
    1:4 Average % 104  
    Range % 99-109  
    1:8 Average % 103  
    Range % 99-106  
  • 回收率：
    The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-102  
    EDTA plasma (n=4) 91 85-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.187 0.183 0.185    
    100 0.259 0.267 0.263    
    25 0.471 0.452 0.462    
    6.25 0.862 0.843 0.853    
    1.56 1.410 1.398 1.404    
    0 2.251 2.159 2.205    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

显示更多
收起更多

产品评价

相关产品

CXCL8 antibodies

CXCL8 Proteins

相关问答

■ 常见问题解答
Q:

Can I get a copy of the kit instructions to as well as any references to papers that have used this kit? I want to make sure it will work in our specific use case. To that end I'm looking for information about the nature of the antibodies (capture and detection) are they monoclonal or poly. Are they the same Ab for both steps or different? Are the plates recoated, or do we need to coat them ourselves? Thank you for the help.

A:
Thanks for your inquiry.
I will send you the manual by e-mail.
Here is the reference about this kit: https://www.sciencedirect.com/science/article/pii/S0165242717302362
The plate is ready to use and you don't need to coat it by yourself. The plate is coated with specific antigen.There is only one antibody in the kit and it is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.
显示更多
收起更多

靶点详情

  • 功能：
    May be an autocrine factor that promotes the growth of fibroblasts and is involved in the neoplastic transformation of fibroblasts by v-Src. Chemotactic for peripheral blood mononuclear cells as well as for heterophils.
  • 基因功能参考文献：
    1. Lower levels in airway aspirates from breastfed infants with acute bronchiolitis PMID: 20337964
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 数据库链接：

    KEGG: gga:396495

    STRING: 9031.ENSGALP00000019051

    UniGene: Gga.826

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |