Chicken Interleukin 4,IL-4 ELISA Kit

Chicken Interleukin 4,IL-4 ELISA Kit

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中文名称：

鸡白介素4(IL-4)酶联免疫试剂盒
货号：

CSB-E06756Ch
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO鸡白介素4（IL-4）酶联免疫检测试剂盒（货号：CSB-E06756Ch），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的IL-4水平。IL-4作为禽类重要的Th2型细胞因子，在免疫调节中发挥关键作用，包括促进B细胞增殖分化、抗体类型转换以及调控过敏反应和抗寄生虫免疫应答，是研究禽类免疫机制的重要靶标。该试剂盒检测线性范围覆盖12.5-800 pg/mL，最低检测灵敏度达1.56 pg/mL，预包被高亲和力抗体确保检测特异性，实验全程可在4.5小时内完成。适用于科研领域对鸡免疫系统功能的研究，如传染病模型中的细胞因子动态分析、疫苗免疫效果评估或免疫相关疾病的分子机制探索。试剂盒含标准品、检测抗体及显色底物等完整组分，提供详细的操作指南，建议短期（1周内）保存于4℃环境，长期保存需置于-20℃且避免反复冻融，有效期为12个月。
缩写：

IL-4
Uniprot No.：

Q5W4U1
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-95
1:2	Average %	94
	Range %	88-101
1:4	Average %	93
	Range %	86-106
1:8	Average %	95
	Range %	82-100

回收率：

The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-97
EDTA plasma (n=4)	92	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	1.726	1.825	1.776	1.665
400	1.336	1.383	1.360	1.249
200	1.060	0.981	1.021	0.910
100	0.714	0.706	0.710	0.599
50	0.481	0.475	0.478	0.367
25	0.307	0.292	0.300	0.189
12.5	0.227	0.214	0.221	0.110
0	0.108	0.113	0.111

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Effects of Escherichia coli strain Nissle 1917 on immune responses of Japanese quails (Coturnix japonica) to Newcastle disease vaccines R Abbasi Kali,Iranian Journal of Veterinary Research,2023
Ameliorative effects of Sida acuta and vitamin C on serum DNA damage, pro-inflammatory and anti-inflammatory cytokines in roosters fed aflatoxin B1 contaminated diets OJ Olarotimi,Olarotimi O J,,2023
Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge Zonghui Zuo,Scientific Reports,2021
Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo Rui Fang,Parasitology Research,2021
Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens Xin Liu,Italian Journal of Animal Science,2021
In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4 P Zhao,Veterinary Parasitology,2020
A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,Journal of Immunology Research,2019
Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation Saleh KMM, et al,Animals,2019
Immunogenic potential and protective efficacy of a sptP deletion mutant of Salmonella Enteritidis as a live vaccine for chickens against a lethal challenge Yaxin Guo, et al,International Journal of Medical Microbiology,2019
The effects and combinational effects of Bacillus subtilis and montmorillonite supplementation on performance, egg quality, oxidation status, and immune response in laying hens J.F.Chen, et al,The Egyptian Journal of Neurology, Psychiatry and Neurosurgery,2019
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene Tingqi Liu.et al,Parasites & Vectors,2018
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
Correlation between vascular endothelial growth factor and subclinical atherosclerosis in patients with psoriasis Shahidi-Dadras M. et al,Int J Dermatol.,2015
Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.