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中文名称：鸡白介素2(IL-2)酶联免疫试剂盒
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货号：CSB-E06755Ch
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：本试剂盒专为定量检测鸡血清、血浆及组织匀浆样本中的白介素2（IL-2）设计，采用双抗体夹心酶联免疫吸附法，检测灵敏度达0.18 pg/mL，线性范围为0.18-12 pg/mL，适用于科研领域对IL-2的精准定量分析。IL-2作为T淋巴细胞分泌的关键细胞因子，在免疫调节中发挥核心作用，参与激活NK细胞、促进B细胞分化等重要生物学过程。该试剂盒包含预包被特异性抗体的酶标板、标准品及检测试剂，通过生物素-链霉亲和素放大系统实现目标蛋白的高效捕获与信号检测，操作流程可在4.5小时内完成。科研人员可将其应用于家禽免疫机制研究、疫苗效力评估或疾病模型中细胞免疫状态的动态监测，特别适用于分析感染性疾病、炎症反应及免疫调节实验中IL-2的表达水平变化。试剂盒兼容多种生物样本类型，为实验室开展禽类免疫学研究提供了标准化检测工具。
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缩写：IL-2
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Uniprot No.：
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种属：Gallus gallus (Chicken)
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样本类型：serum, plasma, tissue homogenates
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检测范围：0.18 pg/mL-12 pg/mL
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灵敏度：0.04 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 85-96 1:2 Average % 90 Range % 85-97 1:4 Average % 99 Range % 94-106 1:8 Average % 97 Range % 92-104 -
回收率：
The recovery of chicken IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 100 92-109 EDTA plasma (n=4) 96 90-104 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 12 2.151 2.198 2.175 2.072 6 1.835 1.869 1.852 1.749 3 1.457 1.487 1.472 1.369 1.5 1.098 1.051 1.075 0.972 0.75 0.698 0.682 0.690 0.587 0.38 0.449 0.460 0.455 0.352 0.18 0.236 0.222 0.229 0.126 0 0.102 0.104 0.103 -
数据处理：
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货期：3-5 working days
引用文献
- Influence of Graded Levels of l-Theanine Dietary Supplementation on Growth Performance, Carcass Traits, Meat Quality, Organs Histomorphometry, Blood Chemistry and Immune Response of Broiler Chickens Saeed M,International journal of molecular sciences,2023
- Effects of essential oil/palygorskite composite on performance, egg quality, plasma biochemistry, oxidation status, immune response and intestinal morphology of laying hens H.Cheng,Poultry Science,2021
- Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens Xin Liu,Italian Journal of Animal Science,2021
- Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy T Xing,J. Sci. Food Agric,2020
- A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,Journal of Immunology Research,2019
- Effect of the melatonin nuclear receptor RORα on monochromatic light-induced T-lymphocyte proliferation in chicken thymus Xiong J, et al,Immunology Letters,2019
- The effects and combinational effects of Bacillus subtilis and montmorillonite supplementation on performance, egg quality, oxidation status, and immune response in laying hens J.F.Chen, et al,The Egyptian Journal of Neurology, Psychiatry and Neurosurgery,2019
- Effects of Supplementing Laying Hens with Purified Amino Acid Prepared from Animal Blood N. Joshi, et al,Tropical animal science journal,2019
- Effects of three probiotic Bacillus on growth performance, digestive enzyme activities, antioxidative capacity, serum immunity, and biochemical parameters in broilers Li Gong.et al,animal science journal,2018
- Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,2018
- The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
- Effects of modified montmorillonite adsorbent on performance, egg quality, serum biochemistry, oxidation status, and immune response of laying hens in late Qu, X. Y.et al,Livestock Science,2018
- The effect of heat stress on intestinal integrity and Salmonella invasion in broiler birds Alhenaky A.et al,J Therm Biol.,2017
- Dietary Supplementation of Phoenix dactylifera Seeds Enhances Performance, Immune Response, and Antioxidant Status in Broilers Ali H. El-Far.et al,Oxidative Medicine and Cellular Longevity,2016
- Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,2016
- The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
- The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015
- Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,Can J Vet Res.,2015
- Omega-3 polyunsaturated fatty acids enrichment alters performance and immune response in infectious bursal disease challenged broilers Elham Maroufyan et al,Lipids in Health and Disease,2012
- Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein Hassan Moeini et al,Virology Journal,2011
- Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus Hassan Moeini et al,Tumor Biology,2010
- Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
- Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015
- Consumption of Oxidized Soybean Oil Increased Intestinal Oxidative Stress and Affected Intestinal Immune Variables in Yellow-feathered Broilers Fangfang Liang. et al,Asian Australas. J. Anim. Sci. ,2015
- Development of a DNA vaccine against chicken anemia virus by using a bicistronic vector expressing VP1 and VP2 proteins of CAV /,Comparative Immunology, Microbiology and Infectious Diseases,2011
- Bursopentine as a Novel Immunoadjuvant Enhances both Humoral and Cell-Mediated Immune Responses to Inactivated H9N2 Avian Influenza Virus in Chickens /,clinical and vaccine immunology,2011
产品评价
靶点详情
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最新研究进展：IL-2是一种细胞因子，可刺激T细胞和自然杀伤细胞的增殖和活化。最新研究表明，IL-2在癌症和免疫治疗方面具有潜在的应用价值。例如，IL-2在肝癌和结直肠癌等方面的临床应用已经得到研究。此外，IL-2还被用于免疫治疗，如通过IL-2增强T细胞治疗肿瘤的方法。
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