Chicken Interleukin 18,IL-18 ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO鸡白介素18（IL-18）酶联免疫检测试剂盒（货号CSB-E10070Ch），采用双抗体夹心法定量检测鸡血清、血浆及组织匀浆样本中IL-18蛋白含量。IL-18是一种参与先天免疫和炎症反应的关键细胞因子，在宿主防御、Th1细胞分化及炎症性疾病调控中发挥重要作用。本试剂盒检测范围为20-500 pg/mL，灵敏度达1.0 pg/mL，实验全程约需3小时，仅需50μL样本即可完成检测。试剂盒包含预包被捕获抗体的96孔板、系列浓度标准品、生物素标记检测抗体、链霉亲和素-HRP复合物及显色底物，操作流程标准化，适用于家禽免疫学研究、疫苗效力评估或感染性疾病模型的建立。科研人员可通过该试剂盒精准分析鸡类样本中IL-18的表达水平，探究其在传染性法氏囊病、新城疫等病原体感染过程中的免疫调控机制，或评估营养干预、环境应激对家禽免疫功能的影响。本产品需2-8℃避光保存，开封后试剂有效期6个月。
别名：

IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit
缩写：

IL18
Uniprot No.：

Q8QFQ8
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue Homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-102
1:2	Average %	90
	Range %	82-99
1:4	Average %	93
	Range %	85-102
1:8	Average %	96
	Range %	92-100

回收率：

The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	81-99
EDTA plasma (n=4)	95	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

STRING: 9031.ENSGALP00000012772

UniGene: Gga.17593