Chicken Interleukin 12,IL-12/70 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO鸡白介素12（IL-12/70）定量检测试剂盒（货号：CSB-E12836C），基于竞争法原理开发，适用于科研领域对血清、血浆及组织匀浆样本中IL-12的精准检测。IL-12是主要由抗原呈递细胞分泌的关键细胞因子，通过激活T细胞和NK细胞参与先天性与适应性免疫调控，在禽类感染免疫及炎症反应研究中具有重要意义。该试剂盒采用预包被抗体与样本中IL-12竞争结合生物素标记抗原的检测体系，可在0.78-200 pg/mL线性范围内实现高灵敏度定量分析，最低检测限达0.78 pg/mL，满足低丰度样本的检测需求。实验全程可在4小时内完成，仅需微量样本（50-100 μL）即可完成检测，适用于家禽免疫机制研究、疫苗效力评估、病原感染模型中的细胞因子动态监测等科研场景，为禽类免疫相关疾病的分子机制探索提供可靠工具。试剂盒包含标准品、预包被板及检测所需全套试剂组分，支持-20℃长期保存，建议实验前通过标准曲线验证批次稳定性。
缩写：

IL-12/70
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-12/70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-98
1:2	Average %	94
	Range %	90-98
1:4	Average %	92
	Range %	87-98
1:8	Average %	96
	Range %	92-101

回收率：

The recovery of chicken IL-12/70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	92	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.